Effect of two dehydration processes on extracts from Opuntia atropes and characterization of polyphenolic compounds by ultra high-resolution liquid chromatograph coupled with mass spectrometry
| Autor: | Héctor Eduardo Martínez-Flores, José Octavio Rodiles-López, Eunice Tranquilino-Rodríguez, Guillermo C. G. Martínez-Ávila |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lignan
ABTS Chromatography DPPH Nutrition. Foods and food supply General Chemical Engineering antioxidant activity dehydration General Chemistry TP368-456 Industrial and Manufacturing Engineering Food processing and manufacture uplc-q/tof-ms2 chemistry.chemical_compound chemistry Polyphenol opuntia atropes TX341-641 Gallic acid polyphenolic profile Quercetin Kaempferol nano-spray Isorhamnetin Food Science |
| Zdroj: | CyTA-Journal of Food, Vol 19, Iss 1, Pp 805-813 (2021) |
| ISSN: | 1947-6345 1947-6337 |
| Popis: | espanolEl objetivo de esta investigacion fue evaluar los compuestos polifenolicos, la actividad antioxidante y la identificacion individual de los diferentes tipos de compuestos fenolicos en extractos secos de Opuntia atropes. El extracto de O. atropes se deshidrato en un horno de secado a 50°C/24 h y mediante secado por nano-pulverizacion a 105°C. El secado por nano-pulverizacion no afecto el contenido de compuestos fenolicos ni la actividad antioxidante del extracto de O. atropes, presentando valores de 11.80 ± 0.61 mg EAG (equivalentes de acido galico)/g de acidos fenolicos, 22.26 ± 1.02 mg EQ (equivalentes de quercetina)/g de flavonoides y porcentaje de inhibicion del radical DPPH• (2,2-difenil-1-picrilhidrazilo) y ABTS• + (acido 2, 2ʹ Azinobis-3-etil- benzotiazolin-6 sulfonico) de 17.92 ± 0.31 y 54.08 ± 0.48, respectivamente. En los extractos en polvo de O. atropes, se identificaron principalmente los glucosidos de kaempferol e isoramnetina mediante cromatografia liquida de ultra alta resolucion acoplada a espectrometria de masas. El lignano de mediorresinol se identifico por primera vez en esta especie de Opuntia. EnglishThe objective of this research was to evaluate the polyphenolic compounds, the antioxidant activity, and the individual identification of the different types of phenolic compounds in dried extracts of Opuntia atropes. O. atropes extract was dehydrated in a drying oven at 50°C/24 h and by nano-spray drying at 105°C. Drying by nano-spray did not affect the content of phenolic compounds or the antioxidant activity of the O. atropes extract, presenting values of 11.80 ± 0.61 mg of gallic acid equivalent/g of phenolic acids, 22.26 ± 1.02 mg quercetin equivalent/g of flavonoids and inhibition percentage of the radical DPPH• (2,2-. diphenil-1-picrilhidrazil) and ABTS•+ (2,2ʹAzinobis(3-ethylbenzothiazoline-6 sulfonic acid)) of 17.92 ± 0.31 and 54.08 ± 0.48, respectively. In the powdered extracts of O. atropes, glycosides of kaempferol and isorhamnetin were mainly identified by ultra high-performance liquid chromatography coupled to mass spectrometry. Mediorresinol lignan was identified for the first time in this Opuntia species. |
| Databáze: | OpenAIRE |
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