Comparative whole genome transcriptome analysis and fenugreek leaf extract modulation on cadmium-induced toxicity in liver cells
| Autor: | Marijo Kent‑First, Caroline O. Odewumi, Roy Leonard Lyles, Lekan M. Latinwo, Cobb‑Abdullah Ahkinyala, Veera L.D. Badisa |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Trigonella
cadmium Cell Survival Cell Cadmium chloride Protective Agents Cell Line Transcriptome rat liver cells 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Genetics medicine Animals Humans Viability assay 030304 developmental biology 0303 health sciences biology Chemistry Plant Extracts General Medicine Articles fenugreek leaf extract biology.organism_classification protection Molecular biology Rats Plant Leaves medicine.anatomical_structure whole genome transcriptome analysis Cell culture Apoptosis Cytoprotection 030220 oncology & carcinogenesis Toxicity Hepatocytes |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | Cadmium (Cd), an economically valuable metal, is widely used in various industrial processes. Although it is of economic value, it is hazardous to human health. Cd accumulates in vital organs where it causes various diseases. Natural compounds with chelating or antioxidant properties have been tested to reduce the toxic effect of Cd. The anti-oxidant, anti-diabetic and hypocholesterolemic properties of fenugreek (Trigonella foenum-graecum) leaves make it a candidate for investigation as protective agent against Cd-induced toxicity. In the present study, the protective effects of fenugreek leaf extract (FLE) on cell viability, morphology, and whole genomic transcription in cadmium chloride (CdCl2)-treated rat liver cells were analyzed. The cells were treated with 25 µM CdCl2 alone, or co-treated with 5 µg/ml FLE for 48 h. The co-treated cells were pretreated with FLE for 2 or 4 h, followed by CdCl2 treatment. Genomic transcription analysis was performed in the CdCl2-treated cells following treatment for 6 h. The CdCl2 caused a significant decrease in viability (35.8±4.1%) and morphological distortion of the cells, compared with the untreated control cells; whereas 4 h pretreatment with FLE (5 µg/ml) reversed the Cd-induced morphology alteration and increased the cell viability to 102±3.8%. Genomic transcription analysis of the CdCl2 only-treated cells showed 61 upregulated and 124 downregulated genes, compared with 180 upregulated and 162 downregulated genes in the FLE pretreated cells. Furthermore, 37 and 26% of the affected total genomic genes in the CdCl2 only-treated cells were involved in binding and catalytic activities, respectively, whereas 50 and 20% of the genes in the FLE pretreated cells were involved in binding and catalytic activities, respectively. In conclusion, these results suggested that genome transcriptome modulation may be important in the protective effect of FLE against Cd-induced toxicity in normal rat liver cells. |
| Databáze: | OpenAIRE |
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