The transcriptome of retinal Müller glial cells
| Autor: | Jeffrey M. Trimarchi, Ben B. Sun, Michael B. Stadler, Ashutosh P Jadhav, Botond Roska, Karin Roesch, Connie Cepko |
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| Rok vydání: | 2008 |
| Předmět: |
BAC transgenic mouse
Retinal Ganglion Cells Chromosomes Artificial Bacterial Müller glia Receptor Platelet-Derived Growth Factor alpha molecular markers Recombinant Fusion Proteins Notch signaling pathway Mice Transgenic In situ hybridization Biology Article Retina Transcriptome Mice Retinal Rod Photoreceptor Cells medicine Animals Cell Lineage RNA Messenger Eye Proteins Oligonucleotide Array Sequence Analysis Integrases Gene Expression Profiling Stem Cells General Neuroscience Molecular biology Cell biology Mice Inbred C57BL Gene expression profiling Amacrine Cells medicine.anatomical_structure nervous system Organ Specificity Neuroglia sense organs Stem cell microarray transcriptome Muller glia murine retina |
| Zdroj: | The Journal of Comparative Neurology |
| ISSN: | 1096-9861 0021-9967 |
| DOI: | 10.1002/cne.21730 |
| Popis: | Müller glial cells are the major type of glia in the mammalian retina. To identify the molecular machinery that defines Müller glial cell identity and function, single cell gene expression profiling was performed on Affymetrix microarrays. Identification of a cluster of genes expressed at high levels suggests a Müller glia core transcriptome, which likely underlies many of the functions of Müller glia. Expression of components of the cell cycle machinery and the Notch pathway, as well as of growth factors, chemokines, and lipoproteins might allow communication between Müller glial cells and the neurons that they support, including modulation of neuronal activity. This approach revealed a set of transcripts that were not previously characterized in (Müller) glia; validation of the expression of some of these genes was performed by in situ hybridization. Genes expressed exclusively by Müller glia were identified as novel markers. In addition, a novel BAC transgenic mouse that expresses Cre in Müller glia cells was generated. The molecular fingerprint of Müller glia provides a foundation for further studies of Müller glia development and function in normal and diseased states. |
| Databáze: | OpenAIRE |
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