First confirmation of Bordetella pertussis occurrence in Slovakia by using real-time PCR
| Autor: | Margita Obernauerova, Cyril Klement, Desana Kohutova, Katarina Tinakova, Ludmila Krajcikova, Lucia Mad'arová |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Bordetella pertussis Slovakia Adolescent Whooping Cough Polymerase Chain Reaction Microbiology law.invention law Medicine Humans Whooping cough Polymerase chain reaction biology business.industry Public Health Environmental and Occupational Health General Medicine biology.organism_classification medicine.disease Biological materials Real-time polymerase chain reaction Child Preschool Female Tos ferina business |
| Zdroj: | Central European journal of public health. 15(1) |
| ISSN: | 1210-7778 |
| Popis: | By application of the real-time PCR we manage to confirm the diagnosis and occurrence of a disease, which is caused by Bordetella pertussis - pertussis. Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS481 genome sequence of Bordetella pertussis was confirmed. This method of detection of pathogens seems to be very rapid, simple, and specific. In the case of adequate technical laboratory equipment it may become very suitable and important supporter in explanation and confirmation of the occurrence of bacterial infections. |
| Databáze: | OpenAIRE |
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