FGF2-responsive genes in human dental pulp cells assessed using a rat spinal cord injury model
| Autor: | Hitomi Aoki, Takahiro Miwa, Ken Sugiyama, Takahiro Kunisada, Toshiyuki Shibata, Yuta Shimizu, Kosuke Nagashima, Hidefumi Fukumitsu, Ken-ichi Tezuka, Kazuki Iida, Naritaka Tamaoki, Tomoko Kawaguchi, Chikara Abe, Hironobu Morita, Daijiro Hatakeyama |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Nervous system MMP1 Endocrinology Diabetes and Metabolism Central nervous system 030209 endocrinology & metabolism Motor Activity Biology Fibroblast growth factor Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Endocrinology Downregulation and upregulation Gene expression medicine Animals Humans Orthopedics and Sports Medicine Spinal cord injury Dental Pulp Spinal Cord Injuries Mesenchymal stem cell Recovery of Function General Medicine medicine.disease Electrophysiological Phenomena Rats Disease Models Animal medicine.anatomical_structure Gene Expression Regulation Cancer research Fibroblast Growth Factor 2 030101 anatomy & morphology |
| Zdroj: | Journal of Bone and Mineral Metabolism. 37:467-474 |
| ISSN: | 1435-5604 0914-8779 |
| Popis: | The central nervous system in adult mammals does not heal spontaneously after spinal cord injury (SCI). However, SCI treatment has been improved recently following the development of cell transplantation therapy. We recently reported that fibroblast growth factor (FGF) 2-pretreated human dental pulp cells (hDPCs) can improve recovery in a rat model of SCI. This study aimed to investigate mechanisms underlying the curative effect of SCI enhanced via FGF2 pretreatment; we selected three hDPC lines upon screening for the presence of mesenchymal stem cell markers and of their functionality in a rat model of SCI, as assessed using the Basso, Beattie, and Bresnahan score of locomotor functional scale, electrophysiological tests, and morphological analyses. We identified FGF2-responsive genes via gene expression analyses in these lines. FGF2 treatment upregulated GABRB1, MMP1, and DRD2, which suggested to contribute to SCI or central the nervous system. In an expanded screening of additional lines, GABRB1 displayed rather unique and interesting behavior; two lines with the lowest sensitivity of GABRB1 to FGF2 treatment displayed an extremely minor effect in the SCI model. These findings provide insights into the role of FGF2-responsive genes, especially GABRB1, in recovery from SCI, using hDPCs treated with FGF2. |
| Databáze: | OpenAIRE |
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