Mechanical stress is communicated between different cell types to elicit matrix remodeling
| Autor: | Roger D. Kamm, Melody A. Swartz, Jeffrey M. Drazen, Daniel J. Tschumperlin |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Immediate-Early Proteins
Signal Transduction/*physiology Cell division Cell Communication Matrix (biology) Matrix metalloproteinase P.H.S DNA-Binding Proteins/biosynthesis Extracellular matrix Bronchi/cytology Lung Transcription Factors/biosynthesis Cells Cultured Cultured Multidisciplinary biology Fibronectins/biosynthesis Biological Sciences Respiratory Mucosa/cytology Epithelial Cells/metabolism Extracellular Matrix Cell biology DNA-Binding Proteins Matrix Metalloproteinase 9 Tissue Inhibitor of Metalloproteinase-1/biosynthesis Collagen Cell Communication/*physiology Cell Division Signal Transduction Cell signaling Cell type Cells Gelatinase B/biosynthesis Bronchi Respiratory Mucosa Fibroblasts/cytology/drug effects/metabolism Collagen/biosynthesis Research Support Stress Immediate early protein Lung/cytology Humans Early Growth Response Protein 1 Tissue Inhibitor of Metalloproteinase-1 Epithelial Cells Fibroblasts Mechanical Coculture Techniques Fibronectins Fibronectin Extracellular Matrix/metabolism/*physiology Immunology biology.protein Coculture Techniques/methods U.S. Gov't Stress Mechanical Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 98:6180-6185 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.111133298 |
| Popis: | Tissue remodeling often reflects alterations in local mechanical conditions and manifests as an integrated response among the different cell types that share, and thus cooperatively manage, an extracellular matrix. Here we examine how two different cell types, one that undergoes the stress and the other that primarily remodels the matrix, might communicate a mechanical stress by using airway cells as a representative in vitro system. Normal stress is imposed on bronchial epithelial cells in the presence of unstimulated lung fibroblasts. We show that ( i ) mechanical stress can be communicated from stressed to unstressed cells to elicit a remodeling response, and ( ii ) the integrated response of two cell types to mechanical stress mimics key features of airway remodeling seen in asthma: namely, an increase in production of fibronectin, collagen types III and V, and matrix metalloproteinase type 9 (MMP-9) (relative to tissue inhibitor of metalloproteinase-1, TIMP-1). These observations provide a paradigm to use in understanding the management of mechanical forces on the tissue level. |
| Databáze: | OpenAIRE |
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