Molecular characterization of the OspA161–175 T cell epitope associated with treatment-resistant Lyme arthritis: differences among the three pathogenic species of Borrelia burgdorferi sensu lato
Autor: | Lisa J. Glickstein, Allen C. Steere, Elise E. Drouin |
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Rok vydání: | 2004 |
Předmět: |
Lipoproteins
T-Lymphocytes Molecular Sequence Data Immunology Epitopes T-Lymphocyte Spirochaetaceae Lymphocyte Activation Lyme Arthritis Borrelia afzelii medicine.disease_cause Epitope Microbiology Lyme disease Drug Resistance Bacterial medicine Humans Immunology and Allergy Amino Acid Sequence Borrelia burgdorferi Cells Cultured Cell Proliferation Arthritis Infectious Lyme Disease biology Immunogenicity bacterial infections and mycoses biology.organism_classification medicine.disease Virology Peptide Fragments Amino Acid Substitution Antigens Surface Bacterial Vaccines Cytokines Borrelia garinii Sequence Alignment Bacterial Outer Membrane Proteins |
Zdroj: | Journal of Autoimmunity. 23:281-292 |
ISSN: | 0896-8411 |
Popis: | Treatment-resistant Lyme arthritis, which may result from infection-induced autoimmunity, is associated with reactivity to a T cell epitope of outer-surface protein A (OspA 161–175 ) of Borrelia burgdorferi sensu stricto ( Bb ). This syndrome has been noted primarily in the United States where only Bb is present, and rarely in Europe where Borrelia garinii ( Bg ) and Borrelia afzelii ( Ba ) predominate. To gain a better understanding of this epitope, we identified its species-specific polymorphisms, determined their immunogenicity, and characterized the contribution of individual amino acids. Based on published sequences the Bb peptide differed from the Ba peptide in six of the nine core residues (amino acids 165–173), whereas the Bg peptide usually differed in three of the nine residues. Lymphocytes from seven patients with treatment-resistant Lyme arthritis proliferated in response to the Bb peptide, but not to the Ba or Bg peptide. Substitution analysis showed that valine 166 and threonine 172 were critical for the immunogenicity of the Bb peptide. Thus, consistent with the geographic distribution of the illness, the European causative agents of Lyme borreliosis usually lack the putative pathogenic OspA epitope. These observations are consistent with the hypothesis that T cell recognition of this epitope is important in the induction of autoimmunity in treatment-resistant Lyme arthritis. |
Databáze: | OpenAIRE |
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