Estimation of Genetic Diversity Using SSR Markers in Sunflower

Autor: Hafeez Ahmad Sadaqat, David J Hole, Bushra Sadia, Z. U. Zia, Waqas Malik, Muhammad Hammad Nadeem Tahir, L. Michaels, B. S. Bushman
Rok vydání: 2014
Předmět:
Zdroj: Генетика. 50:570-580
ISSN: 0016-6758
DOI: 10.7868/s0016675814050142
Popis: Microsatellites or simple sequence repeats (SSRs) were used for the estimation of genetic diversity among a group of 40 sunflower lines developed at the research area of Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad. Total numbers of alleles amplified by 22 polymorphic primers were 135 with an average of 6.13 alleles per locus, suggesting that SSR is a powerful technique for assessment of genetic diversity at molecular level. The expected heterozygosity (PIC) ranged from 0.17 to 0.89. The highest PIC value was observed at the locus C1779. The genetic distances ranged from 9 to 37%. The highest genetic distance was observed between the lines L50 and V3. Genetic distances were low showing lesser amount of genetic diversity among the sunflower lines.
Databáze: OpenAIRE