Covalent attachment of DNA to glass supports using a new silane coupling agent and chemiluminescent detection
| Autor: | Wu Xiaoyan, Zhou Yikai, Yuan Jinwei, Ren Shu, Zhang Guojun |
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| Rok vydání: | 2000 |
| Předmět: |
Detection limit
biology Chemistry Nucleic Acid Hybridization DNA Silanes Combinatorial chemistry Horseradish peroxidase law.invention Nucleic acid thermodynamics chemistry.chemical_compound Covalent bond law Management of Technology and Innovation Luminescent Measurements Triethoxysilane Silicone Elastomers Nucleic acid biology.protein Humans Organic chemistry Glass Horseradish Peroxidase Chemiluminescence |
| Zdroj: | Journal of Tongji Medical University. 20:89-91 |
| ISSN: | 0257-716X |
| Popis: | A new kind of silane coupling agent, N-(beta-aminoethyl)-gamma-aminopropyl triethoxysilane, was used for DNA direct attachment on the surfaces of glass supports, then the immobilized DNA was hybridized with horseradish peroxidase (HRP)-labeled probe, and detected by using enhanced chemiluminescent method. In comparison with gamma-aminopropyl triethoxysilane, the detection limits (S/N) of DNA were 10 pg and 75 pg respectively. Several experimental conditions of DNA attach-ING to glass supports were investigated, and the system of hybridization of nucleic acid on the surfaces of glass supports was developed. |
| Databáze: | OpenAIRE |
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