AMPA and NMDA glutamate receptors are found in both peptidergic and non-peptidergic primary afferent neurons in the rat
| Autor: | Helen H. Willcockson, Juli G. Valtschanoff |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Histology
Substance P AMPA receptor Biology Receptors N-Methyl-D-Aspartate Article Pathology and Forensic Medicine Rats Sprague-Dawley chemistry.chemical_compound Dorsal root ganglion Ganglia Spinal medicine Noxious stimulus Animals Neurons Afferent Receptors AMPA Receptors Purinergic P2 Glutamate receptor Cell Biology Rats medicine.anatomical_structure nervous system chemistry Neuropathic pain Nociceptor NMDA receptor Neuroscience Receptors Purinergic P2X3 |
| Zdroj: | Cell and Tissue Research. 334:17-23 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-008-0662-0 |
| Popis: | Two distinct classes of nociceptive primary afferents, peptidergic and non-peptidergic, respond similarly to acute noxious stimulation; however the peptidergic afferents are more likely to play a role in inflammatory pain, while the non-peptidergic afferents may be more characteristically involved in neuropathic pain. Using multiple immunofluorescence, we determined the proportions of neurons in the rat L4 dorsal root ganglion (DRG) that co-express AMPA or NMDA glutamate receptors and markers for the peptidergic and non-peptidergic classes of primary afferents, substance P and P2X(3), respectively. The fraction of DRG neurons immunostained for the NR1 subunit of the NMDA receptor (40%) was significantly higher than that of DRG neurons immunostained for the GluR2/3 (27%) or the GluR4 (34%) subunits of the AMPA receptor. Of all DRG neurons double-immunostained for glutamate receptor subunits and either marker for peptidergic and non-peptidergic afferents, a significantly larger proportion expressed GluR4 than GluR2/3 or NR1 and in a significantly larger proportion of P2X(3)- than SP-positive DRG neurons. These observations support the idea that nociceptors, involved primarily in the mediation of neuropathic pain, may be presynaptically modulated by GluR4-containing AMPA receptors. |
| Databáze: | OpenAIRE |
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