TMT-based proteomics analysis reveals the protective effects of Xuefu Zhuyu decoction in a rat model of traumatic brain injury
| Autor: | Yang Hang, Shi-Chao Guo, Pengfei Li, Tao Liu, Zian Xia, Jia-Miao Liu, Teng Li, Dan Zhou, Yang Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Proteomics Traumatic brain injury Quantitative proteomics Traditional Chinese medicine Pharmacology Tandem mass tag Cell morphology Rats Sprague-Dawley 03 medical and health sciences symbols.namesake 0302 clinical medicine Brain Injuries Traumatic Drug Discovery medicine Animals KEGG 030304 developmental biology 0303 health sciences business.industry Computational Biology medicine.disease Rats Disease Models Animal 030220 oncology & carcinogenesis Nissl body symbols business Drugs Chinese Herbal Signal Transduction |
| Zdroj: | Journal of Ethnopharmacology. 258:112826 |
| ISSN: | 0378-8741 |
| Popis: | Xuefu Zhuyu decoction (XFZYD) is a traditional Chinese herbal prescription. It is effective in treating traumatic brain injury (TBI). However, the underlying molecular mechanisms remain unclear.This study aimed to reveal the possible mechanisms of XFZYD in treating acute TBI through proteomics clues.Controlled Cortical Impact (CCI) rats were given gavage administration of XFZYD (9 g/kg/d) or distilled water (equal volume) for three days. The Modified Neurological Severity Score (mNSS), brain water content, HE staining, Nissl staining and immunohistochemistry were performed to assess the effects of XFZYD for TBI treatment. Additionally, tandem mass tag-based (TMT) quantitative proteomics technology was applied to detect proteins of brain cortex. Bioinformatics analysis including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and Protein-protein interaction (PPI) networks were used to analyze differentially expressed proteins (DEPs). Bioinformatics Analysis Tool for Molecular mechanism of TCM (BATMAN-TCM) was conducted to anchor diseases and pathways. Besides, western blotting and immunofluorescence were exerted to verify related proteins.XFZYD improved neurologic functions, reduced encephaledema and ameliorated cell morphology around the injured area in CCI rats. A total of 6099 proteins were identified with false discovery rate (FDR) 1%. Overlapping DEPs (105 DEPs) were identified (295 DEPs and 804 DEPs in CCI/Sham or XFZYD/CCI group, respectively). Of these DEPs, 17 were regulated by XFZYD. Bioinformatics analysis showed that the 17 DEPs were predominantly related to platelet activation and PI3K-Akt signaling pathway. Next, PLG and CD34 were verified with molecular biotechnology.XFZYD exerts therapeutic effects through multi-pathways regulation in the treatment of TBI. This work may provide proteomics clues for the continuation of research on TBI treatment with XFZYD. |
| Databáze: | OpenAIRE |
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