Cellular immune responses in human immunodeficiency virus (HIV)-1-infected children: is immune restoration by highly active anti-retroviral therapy comparable to non-progression?
Autor: | Liliane Schandené, Marc Hainaut, Martine Ducarme, Jack Levy, Virginie Verscheure, Françoise Mascart |
---|---|
Rok vydání: | 2011 |
Předmět: |
Male
Cellular immunity Adolescent Translational Studies medicine.medical_treatment T cell T-Lymphocytes Immunology HIV Core Protein p24 HIV Infections Lymphocyte Activation Disease-Free Survival Young Adult Immune system Antigen Interferon Immunopathology Antiretroviral Therapy Highly Active medicine Immunology and Allergy Humans Child Th1-Th2 Balance Cells Cultured Phytohaemagglutinin Cell Proliferation biology virus diseases Infant Virology Infectious Disease Transmission Vertical Cytokine medicine.anatomical_structure Child Preschool biology.protein HIV-1 Cytokines Female medicine.drug |
Zdroj: | Clinical and experimental immunology. 165(1) |
ISSN: | 1365-2249 |
Popis: | Summary The objective of this study was to investigate whether the restored immune functions of vertically human immunodeficiency virus (HIV)-infected children who were severely immunodeficient before the initiation of highly active anti-retroviral therapy (HAART) are comparable to those of untreated slow progressors. We therefore assessed T cell proliferation and cytokine [interferon (IFN)-γ, interleukin (IL)-5 and IL-13] secretions after mitogen, recall antigens and HIV-1-specific stimulation in 12 untreated slow progressors, 16 untreated progressors and 18 treated patients. Treated children were profoundly immunodeficient before the initiation of HAART and had long-lasting suppression of viral replication on treatment. We demonstrated that slow progressors are characterized not only by the preservation of HIV-1-specific lymphoproliferative responses but also by the fact that these responses are clearly T helper type 1 (Th1)-polarized. Children on HAART had proliferative responses to HIV-1 p24 antigen, purified protein derivative (PPD) and tetanus antigen similar to slow progressors and higher than those of progressors. However, in contrast to slow progressors, most treated children exhibited a release of Th2 cytokines accompanying the IFN-γ secretion in response to the HIV-1 p24 antigen. Moreover, despite higher proliferative responses to phytohaemagglutinin (PHA) than the two groups of untreated children, treated children had lower levels of IFN-γ secretion in response to PHA than slow progressors. These data show that in severely immunodeficient vertically HIV-infected children, a long-lasting HAART allows recovering lymphoproliferative responses similar to untreated slow progressors. However, alterations in IFN-γ secretion in response to the mitogen PHA persisted, and their cytokine release after HIV-specific stimulation was biased towards a Th2 response. |
Databáze: | OpenAIRE |
Externí odkaz: |