Molecular data supports monophyly of Triatoma dispar complex within genus Triatoma

Autor: Mario J. Grijalva, Soledad Santillán-Guayasamín, Ezequiel Magallón-Gastélum, Etienne Waleckx, César A. Yumiseva, Christian Barnabé, Anita G. Villacís, Simone Frédérique Brenière
Přispěvatelé: Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB)
Rok vydání: 2020
Předmět:
0301 basic medicine
Microbiology (medical)
Dispar
030106 microbiology
Genes
Insect
Tribe (biology)
Microbiology
DNA
Mitochondrial
Electron Transport Complex IV
03 medical and health sciences
Monophyly
Genus
RNA
Ribosomal
16S
parasitic diseases
DNA
Ribosomal Spacer
Genetics
RNA
Ribosomal
18S
Animals
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Chagas Disease
Triatoma
Molecular Biology
Ecology
Evolution
Behavior and Systematics
Phylogeny
biology
Phylogenetic tree
Cytochrome b
Genetic Variation
Bayes Theorem
Central America
Biodiversity
Sequence Analysis
DNA
Cytochromes b
South America
biology.organism_classification
Maximum parsimony
Insect Vectors
030104 developmental biology
Infectious Diseases
Genes
Mitochondrial
Haplotypes
Evolutionary biology
RNA
Ribosomal
North America
Insect Proteins
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Zdroj: Infection, Genetics and Evolution
Infection, Genetics and Evolution, Elsevier, 2020, 85, art. 104429 [9 p.]. ⟨10.1016/j.meegid.2020.104429⟩
ISSN: 1567-7257
1567-1348
Popis: The genus Triatoma contains numerous species, principal or secondary vectors of Chagas disease, which have been included in the three main lineages of Triatomini tribe based on morphological and biogeographical characteristics: North American, South American, and T. dispar complex. The three members of the T. dispar complex are distributed in Ecuador. This complex has been scarcely studied through molecular approaches, and the taxonomic position of this complex is not confirmed. In this study, we explored the phylogenetic relationships within the genus Triatoma, including five species from North and Central America, six from South America, and the three species belonging to the T. dispar complex. Partial sequences of four mitochondrial genes (Cyt b, COII, 16S-rRNA, 12S-rRNA) and two nuclear genes (18S-rRNA, ITS2) were obtained from 74 specimens. Phylogenetic trees were built with concatenated and single sequences through maximum likelihood (ML), maximum parsimony (MP), and Bayesian methods. The trees built using concatenated sequences showed three main branches (clusters) highly supported by significant bootstrap values; the T. dispar complex appeared as a monophyletic group separate from species of North and Central American origin and South American origin. On the contrary, for each gene tree, the three main clusters were not always significantly supported, mostly because genetic information is dramatically reduced when a single gene is considered. Consequently, concatenation of genes gives relevant results and is highly recommended for further in-depth examination of the relationships of several species and complexes of triatomines that remain unresolved. Moreover, our current molecular data fully revealed the division of genus Triatoma into at least three main genetic groups.
Databáze: OpenAIRE
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