Pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the paired serum and cerebrospinal fluid of children
| Autor: | Tomoyuki Akiyama, Katsuhiro Kobayashi, Mari Akiyama, Yoshiyuki Hanaoka, Tokito Yamaguchi, Takashi Shibata, Yumiko Hayashi, Hiroki Tsuchiya |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pyridoxal 5-Phosphate Pyridoxal Pyridoxic Acid Adolescent Clinical Biochemistry chemical and pharmacologic phenomena Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cerebrospinal fluid immune system diseases Humans Pyridoxal phosphate Child Derivatization Detection limit Semicarbazide Chromatography Biochemistry (medical) Infant Newborn Infant General Medicine nervous system diseases 030104 developmental biology chemistry Child Preschool Pyridoxal Phosphate Female lipids (amino acids peptides and proteins) 030217 neurology & neurosurgery |
| Zdroj: | Clinica Chimica Acta. 472:118-122 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/j.cca.2017.07.032 |
| Popis: | We quantified pyridoxal 5'-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in paired serum and cerebrospinal fluid (CSF) samples from children and investigated the effect of age on the concentrations and CSF-to-serum ratios of these vitamers.Serum and CSF samples prospectively collected from 49 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age on these vitamers, the PLP-to-PL ratio, CSF-to-serum PLP ratio, and CSF-to-serum PL ratio were evaluated using correlation analysis.The PLP, PL, and PA concentrations in the serum and CSF were higher at younger ages, except for CSF PA concentrations that were mostly below the limit of detection (1.2nmol/l). The PLP-to-PL ratios in the serum and CSF correlated positively with age. The CSF-to-serum PLP ratio and CSF-to-serum PL ratio were independent of age.Age-related changes in PLP, PL, and PA in serum and in CSF from pediatric patients and CSF-to-serum ratios of PLP and PL demonstrated in this study will provide valuable information for evaluating PLP supply to the central nervous system from the peripheral blood. |
| Databáze: | OpenAIRE |
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