Immunohistochemical distribution of proteins belonging to the receptor-mediated and the mitochondrial apoptotic pathways in human placenta during gestation
| Autor: | Luigi Cobellis, Valentina Fedele, Vincenza Laforgia, Sebastiano Leone, B. De Luca, D. Giraldi, M. De Falco, A. De Luca, A. Mastrogiacomo |
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| Přispěvatelé: | DE FALCO, Maria, Fedele, V, Cobellis, L, Mastrogiacomo, A, Leone, S, Giraldi, D, DE LUCA, B, Laforgia, Vincenza, DE LUCA, A., DE FALCO, M, Cobellis, Luigi, Laforgia, V, DE LUCA, Antonio |
| Rok vydání: | 2004 |
| Předmět: |
Adult
Cytoplasm Programmed cell death Fas Ligand Protein Histology Nuclear Envelope Immunoelectron microscopy CASP8 and FADD-Like Apoptosis Regulating Protein Apoptosis Gestational Age Biology Fas ligand Pathology and Forensic Medicine Mitochondrial Proteins Pregnancy Placenta medicine Humans fas Receptor Tissue homeostasis Cell Nucleus Membrane Glycoproteins Cytotrophoblast Intracellular Signaling Peptides and Proteins Proteins Cell Biology Trophoblasts Cell biology Apoptotic Protease-Activating Factor 1 medicine.anatomical_structure Caspases Female |
| Zdroj: | Cell and Tissue Research. 318:599-608 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-004-0969-4 |
| Popis: | The balance between cell death and cell proliferation and its regulation are essential features of many physiological processes and are particularly important in fetal morphogenesis and adult tissue homeostasis. Apoptosis is a type of cell suicide that is activated in two main ways: through a receptor-mediated pathway or through a mitochondrial pathway. We have investigated the immunohistochemical distribution of proteins belonging to these two pathways in human placenta during gestation by comparing their expression levels between the first and third trimester of gestation. In the first trimester, the receptor-mediated pathway prevails over the mitochondrial pathway with a moderate/intense expression of its three components, viz., Fas ligand (FasL), Fas, and caspase-8, and weak positivity of anti-apoptotic FLIP, these proteins being mainly localized in the cytotrophoblast compartment. In the third trimester of gestation, there is an increased expression of mitochondrial pathway proteins, viz., Apaf-1 and caspase-9. We have also investigated the expression level of caspase-3, the primary effector caspase of both pathways, and have observed that it is moderately expressed during gestation, being mainly localized in the cytotrophoblast during the first trimester and in both placental compartments during the third trimester of gestation. Thus, both pathways actively function in human placenta to execute cell death. By means of immunoelectron microscopy, we have further shown that, in human placenta, the two proteins of the mitochondrial pathway together with caspase-3 are localized both in the cytoplasm and in the nucleus. In particular, Apaf-1 and caspase-9 are distributed near to the nuclear envelope suggesting an important role for these two proteins in disrupting the nuclear-cytoplasmic barrier. |
| Databáze: | OpenAIRE |
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