Spatiotemporal Dynamics of Re-Innervation and Hyperinnervation Patterns by Uninjured CGRP Fibers in the Rat Foot Sole Epidermis after Nerve Injury
| Autor: | Tom J. H. Ruigrok, Erik T Walbeehm, J.C. Holstege, Liron S. Duraku, Shoista Kambiz, Lukas L Falke, Mehdi Hossaini, Vivek Mudera, S. Hoendervangers |
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| Přispěvatelé: | Plastic and Reconstructive Surgery and Hand Surgery, Neurosciences, Cardiothoracic Surgery, Orthopedics and Sports Medicine |
| Jazyk: | angličtina |
| Předmět: |
Male
Pain Threshold SNi SNI Time Factors Calcitonin Gene-Related Peptide NF-200 Calcitonin gene-related peptide Substance P Cellular and Molecular Neuroscience Nerve Fibers PGP 9.5 Peripheral Nerve Injuries lcsh:Pathology Animals Medicine CGRP Rats Wistar Denervation Staining and Labeling integumentary system Foot business.industry Research Temperature Anatomy Nerve injury Rats Disease Models Animal P2X3 Nociception Anesthesiology and Pain Medicine Molecular Medicine Sciatic nerve medicine.symptom Epidermis business Free nerve ending Common peroneal nerve Evans Blue lcsh:RB1-214 |
| Zdroj: | Molecular Pain, 8. BioMed Central Ltd. Molecular Pain, Vol 8, Iss 1, p 61 (2012) Molecular Pain |
| ISSN: | 1744-8069 |
| DOI: | 10.1186/1744-8069-8-61 |
| Popis: | The epidermis is innervated by fine nerve endings that are important in mediating nociceptive stimuli. However, their precise role in neuropathic pain is still controversial. Here, we have studied the role of epidermal peptidergic nociceptive fibers that are located adjacent to injured fibers in a rat model of neuropathic pain. Using the Spared Nerve Injury (SNI) model, which involves complete transections of the tibial and common peroneal nerve while sparing the sural and saphenous branches, mechanical hypersensitivity was induced of the uninjured lateral (sural) and medial (saphenous) area of the foot sole. At different time points, a complete foot sole biopsy was taken from the injured paw and processed for Calcitonin Gene-Related Peptide (CGRP) immunohistochemistry. Subsequently, a novel 2D-reconstruction model depicting the density of CGRP fibers was made to evaluate the course of denervation and re-innervation by uninjured CGRP fibers. The results show an increased density of uninjured CGRP-IR epidermal fibers on the lateral and medial side after a SNI procedure at 5 and 10 weeks. Furthermore, although in control animals the density of epidermal CGRP-IR fibers in the footpads was lower compared to the surrounding skin of the foot, 10 weeks after the SNI procedure, the initially denervated footpads displayed a hyper-innervation. These data support the idea that uninjured fibers may play a considerable role in development and maintenance of neuropathic pain and that it is important to take larger biopsies to test the relationship between innervation of injured and uninjured nerve areas. |
| Databáze: | OpenAIRE |
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