Inhibition of sialyl transferase activity by gossypol acetic acid in human seminal plasma
| Autor: | M. Sagiv, D. Allalouf, Rina Singer, N. Lehrer, H. Levinsky |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Urology Sodium Population chemistry.chemical_element Andrology chemistry.chemical_compound Endocrinology Semen Degree Celsius Internal medicine Humans Medicine education beta-D-Galactoside alpha 2-6-Sialyltransferase Incubation education.field_of_study Sperm Count business.industry Gossypol General Medicine Sialyltransferases Sialic acid chemistry Benzyl alcohol business Gossypol Acetic Acid |
| Zdroj: | Andrologia. 23:159-161 |
| ISSN: | 1439-0272 0303-4569 |
| DOI: | 10.1111/j.1439-0272.1991.tb02522.x |
| Popis: | Seventeen aliquots of 15 microliters seminal plasma of human origin were incubated with 25 micrograms, 15 micrograms, 5 micrograms and 2.5 micrograms gossypol acetic acid (GAA), dissolved in BWW containing 0.9% sodium chloride and 0.9% benzyl alcohol, at 37 degrees C for 60 minutes. Following incubation, activity of sialyl transferase (S.T.) was determined by a procedure involving incorporation of radioactive sialic acid into asialofetuin. The activity of S.T., expressed as cpm per hour per 15 microliters seminal plasma was compared to controls consisting in incubation of sperm in BWW containing 0.9% benzyl alcohol and 0.9% sodium chloride. The activity of S.T. in controls represented 61.3 +/- 8.0%-67.7 +/- 8.9% of the activity obtained by incubation with BWW only. GAA was found to exert a dose-dependent inhibition of S.T. activity, ranging from 38.3 +/- 20.6% to 53.4 +/- 19.4% (with 25 micrograms) and from 11.3 +/- 14.8% to 21.9 +/- 14.8% (with 2.5 micrograms). |
| Databáze: | OpenAIRE |
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