Mapping a highly conserved linear neutralizing epitope on gD glycoprotein of bovine herpesvirus type I using a monoclonal antibody
| Autor: | Ying Bi, Xin Wang, Xiaobo Wen, Xiaodan Tong, Xuhua Ran, Hongbo Ni |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
gD
040301 veterinary sciences medicine.drug_class Viral pathogenesis Monoclonal antibody Antibodies Viral Epitope law.invention 0403 veterinary science 03 medical and health sciences Epitopes Viral Envelope Proteins Viral entry law Alphaherpesvirinae Virology medicine Escherichia coli Amino Acid Sequence bovine herpesvirus 030304 developmental biology Herpesvirus 1 Bovine epitope map chemistry.chemical_classification 0303 health sciences General Veterinary Linear epitope biology Full Paper Chemistry Antibodies Monoclonal 04 agricultural and veterinary sciences biology.organism_classification Recombinant Proteins monoclonal antibody Recombinant DNA Glycoprotein Epitope Mapping |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 |
| Popis: | Bovine herpesvirus type 1 (BoHV-1), a member of the Alphaherpesvirinae, causes a variety of diseases, which result in significant economic losses worldwide. Envelope glycoprotein D (gD) of BoHV-1 plays an important role in viral entry into the permissive cells, and protective immune response. The fine mapping epitope on the gD will contribute to the understanding of viral pathogenesis and development of alternative vaccines against various diseases associated with BoHV-1. We previously reported the preparation of a monoclonal antibody (MAb) 2B6, which was raised by a truncated recombinant gD protein, demonstrating a neutralizing activity against BoHV-1 infection in Madin-Darby bovine kidney cells. This study described the identification of a linear B-cell epitope on gD using MAb 2B6. A series of partially overlapping gD proteins with glutathione S-transferase tag were generated to define the epitope recognized by MAb 2B6. The amino acid (aa) sequence 323GEPKPGPSPDADRPE337 was recognized by MAb 2B6 using Western blot with the variedly truncated recombinant proteins. Importantly, this epitope was highly conserved among the typical members of BoHV-1, indicating that the epitope may be utilized in diagnosis of diseases due to BoHV-1 infection. Furthermore, the minimal linear epitope sequence 323GEPKPGP329 on gD recognized by MAb 2B6 was confirmed using single-aa residue deletion mutation in carboxyl terminal. This finding not only contributes to our understanding of gD of BoHV-1 virion but also shows a potential for the development of vaccine candidates and diagnostic techniques. |
| Databáze: | OpenAIRE |
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