Transcriptome analysis identifies genes with enriched expression in the mouse central extended amygdala
| Autor: | Doulaye Dembélé, Katia Befort, Muriel Koch, Olivier Poch, Brigitte L. Kieffer, Clara Blad, Christelle Thibault, Aditee Ghate, Jean Muller, Aurélie Lardenois, Jérôme A.J. Becker, Dominique Filliol |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Institut Clinique de la Souris (ICS), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Peney, Maité |
| Rok vydání: | 2008 |
| Předmět: |
Gene Expression
Nerve Tissue Proteins Nucleus accumbens Biology Amygdala Article Transcriptome 03 medical and health sciences Mice 0302 clinical medicine Extended amygdala Gene expression medicine Animals 030304 developmental biology [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM] Oligonucleotide Array Sequence Analysis Genetics 0303 health sciences Brain Mapping [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] General Neuroscience Central nucleus of the amygdala Gene Expression Profiling [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] Cell biology Gene expression profiling Mice Inbred C57BL Stria terminalis medicine.anatomical_structure [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Neuroscience, Elsevier-International Brain Research Organization, 2008, 156 (4), pp.950-65. ⟨10.1016/j.neuroscience.2008.07.070⟩ Neuroscience, 2008, 156 (4), pp.950-65. ⟨10.1016/j.neuroscience.2008.07.070⟩ |
| ISSN: | 0306-4522 1873-7544 |
| Popis: | International audience; The central extended amygdala (EAc) is an ensemble of highly interconnected limbic structures of the anterior brain, and forms a cellular continuum including the bed nucleus of the stria terminalis (BNST), the central nucleus of the amygdala (CeA) and the nucleus accumbens shell (AcbSh). This neural network is a key site for interactions between brain reward and stress systems, and has been implicated in several aspects of drug abuse. In order to increase our understanding of EAc function at the molecular level, we undertook a genome-wide screen (Affymetrix) to identify genes whose expression is enriched in the mouse EAc. We focused on the less-well known BNST-CeA areas of the EAc, and identified 121 genes that exhibit more than twofold higher expression level in the EAc compared with whole brain. Among these, 43 genes have never been described to be expressed in the EAc. We mapped these genes throughout the brain, using non-radioactive in situ hybridization, and identified eight genes with a unique and distinct rostro-caudal expression pattern along AcbSh, BNST and CeA. Q-PCR analysis performed in brain and peripheral organ tissues indicated that, with the exception of one (Spata13), all these genes are predominantly expressed in brain. These genes encode signaling proteins (Adora2, GPR88, Arpp21 and Rem2), a transcription factor (Limh6) or proteins of unknown function (Rik130, Spata13 and Wfs1). The identification of genes with enriched expression expands our knowledge of EAc at a molecular level, and provides useful information to toward genetic manipulations within the EAc. |
| Databáze: | OpenAIRE |
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