Time-dependent efficacy of combination of silver-containing hydroxyapatite coating and vancomycin on methicillin-resistant Staphylococcus aureus biofilm formation in vitro
| Autor: | Iwao Noda, Hiroshi Miyamoto, Takeo Shobuike, Tomoki Kobatake, Masaaki Mawatari, Sakumo Kii, Motoki Sonohata, Akira Hashimoto |
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| Rok vydání: | 2021 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine Silver 030106 microbiology lcsh:Medicine MRSA medicine.disease_cause General Biochemistry Genetics and Molecular Biology Hydroxyapatite Microbiology 03 medical and health sciences Vancomycin medicine Humans lcsh:Science (General) lcsh:QH301-705.5 biology Inoculation Chemistry Biofilm lcsh:R General Medicine Staphylococcal Infections biochemical phenomena metabolism and nutrition biology.organism_classification Methicillin-resistant Staphylococcus aureus In vitro Anti-Bacterial Agents Research Note Durapatite 030104 developmental biology lcsh:Biology (General) Staphylococcus aureus Biofilms Hydroxyapatite coating Bacteria lcsh:Q1-390 medicine.drug |
| Zdroj: | BMC Research Notes BMC Research Notes, Vol 14, Iss 1, Pp 1-5 (2021) |
| ISSN: | 1756-0500 |
| Popis: | Objective We developed a silver-containing hydroxyapatite (Ag-HA) coating to prevent periprosthetic joint infection (PJI). Methicillin-resistant Staphylococcus aureus (MRSA) is the main PJI-causing bacteria. Previously, we had reported the combined effect of Ag-HA coating and vancomycin (VCM) on MRSA biofilm formation 24 h after MRSA inoculation. In this study, we investigated the time-dependent efficacy of Ag-HA coating and VCM on MRSA biofilm formation on Ti discs in vitro by three-dimensional confocal laser scanning microscopic analysis. Results For the Ti VCM and HA VCM groups, the total biofilm volumes per area at 96 h after MRSA inoculation were significantly larger than those at 48 h after MRSA inoculation, respectively (p p p |
| Databáze: | OpenAIRE |
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