Aspartyl proteases from the intestinal brush border of Haemonchus contortus as protective antigens for sheep
| Autor: | Philip Skuce, George F.J. Newlands, D. Pettit, S. K. Smith, W. D. Smith |
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| Rok vydání: | 2003 |
| Předmět: |
Brush border
Molecular Sequence Data Immunology Sheep Diseases Epitope law.invention Epitopes Antigen Glycoprotein complex law Endopeptidases parasitic diseases Animals Aspartic Acid Endopeptidases Amino Acid Sequence chemistry.chemical_classification Membrane Glycoproteins Sheep Base Sequence Microvilli Sequence Homology Amino Acid biology Helminth Proteins DNA Helminth biology.organism_classification Molecular biology Recombinant Proteins Intestines Molecular Weight chemistry Antigens Helminth Recombinant DNA biology.protein Haemonchus Parasitology Antibody Haemonchiasis Glycoprotein Haemonchus contortus |
| Zdroj: | Parasite Immunology. 25:521-530 |
| ISSN: | 1365-3024 0141-9838 |
| DOI: | 10.1111/j.0141-9838.2004.00667.x |
| Popis: | SUMMARY A novel pepsin-like aspartyl protease was identified as a component of Haemonchus galactose-containing glycoprotein (H-gal-GP), which is an integral membrane glycoprotein complex located on the intestinal cells of Haemonchus contortus, and a highly protective antigen for sheep. This molecule, designated HcPEP2, showed 50% sequence identity with a previously described aspartyl protease from H-gal-GP known as HcPEP1. Fractions of H-gal-GP, either containing both HcPEP1 and 2 or other lower molecular weight components of the complex, were evaluated as protective antigens in immunization – challenge trials in sheep. When separated from the rest of the complex by gel filtration in 8 m urea, the HcPEP1 and 2 fraction significantly reduced H. contortus egg counts by 48% and worm numbers by 36%, but the lower molecular weight components were not significantly protective. However, the HcPEP1 and 2 fraction did not protect if electro-eluted from SDS-dissociated H-gal-GP, nor did bacterially expressed recombinant HcPEP1, suggesting that conformational epitopes are important for inducing immunity. |
| Databáze: | OpenAIRE |
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