Enhancement of photodynamic inactivation against Pseudomonas aeruginosa by a nano-carrier approach
| Autor: | Wei-Chi Wu, Chi-Hsien Liu, Bishakh Rout |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Light Cell Survival Swine medicine.medical_treatment 030106 microbiology Photodynamic therapy 02 engineering and technology medicine.disease_cause Absorbance Mice 03 medical and health sciences Colloid and Surface Chemistry medicine Zeta potential Animals Microemulsion Tolonium Chloride Physical and Theoretical Chemistry Skin B-Lymphocytes Drug Carriers Hybridomas Microscopy Confocal Photosensitizing Agents Aqueous solution Chemistry Pseudomonas aeruginosa Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Antimicrobial Anti-Bacterial Agents Nanostructures Microscopy Electron Light intensity Animals Newborn Biochemistry Spectrophotometry Biophysics Emulsions 0210 nano-technology Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 140:472-480 |
| ISSN: | 0927-7765 |
| Popis: | As pathogens steadily develop resistance to widely used antibiotics, new methodologies for their efficient inactivation must be developed. Photodynamic therapy is an upcoming technique that provides an alternative option for treating pathogenic infections. The efficiency of photodynamic therapy has been limited by the use of aqueous mediums for dispersing photosensitising agents. Toluidine Blue O (TBO) was chosen for this study as a cationic photosensitiser to inhibit Gram-negative bacterium Pseudomonas aeruginosa. Enhanced delivery of the photosensitiser was ensured by utilising an essential oil-based microemulsion. The efficiency of photodynamic therapy was further improved by the use of a chemical penetration enhancer to improve permeability of the bacterial outer membrane. TBO accumulation patterns in neonate pig skin were studied using confocal laser scanning microscopy. The physicochemical properties of the TBO loaded microemulsion, including UV-vis absorbance, size distribution and zeta potential, were analysed to understand the enhanced antimicrobial activity. Confocal laser scanning microscopy confirmed the formation of a TBO reservoir in the skin by the TBO-loaded microemulsions. TBO (5 μg/mL) in the vehicles significantly inhibited the growth of P. aeruginosa. All these efforts resulted in inhibition obtained at a drug concentration and light intensity much lower than what is reported by the works of previous investigators. |
| Databáze: | OpenAIRE |
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