Selection and molecular characterization of Bacillus thuringiensis strains efficient against soybean looper (Chrysodeixis includens) and Spodoptera species
| Autor: | Fernando Hercos Valicente, Tatiane Aparecida Nascimento Barbosa, Karine Silva de Carvalho, Ubiraci Gomes de Paula Lana |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Larva
Multiple pest control biology Molecular approach business.industry fungi General Engineering Pest control food and beverages Genetically modified crops Spodoptera biology.organism_classification Lepidoptera Lepidoptera genitalia B. thuringiensis QL1-991 Chrysodeixis includens Bacillus thuringiensis Botany Bioassay business Zoology |
| Zdroj: | Revista Brasileira de Entomologia, Volume: 64, Issue: 4, Article number: e20200080, Published: 16 DEC 2020 Revista Brasileira de Entomologia, Vol 64, Iss 4 (2020) Revista Brasileira de Entomologia v.64 n.4 2020 Revista brasileira de entomologia Sociedade Brasileira De Entomologia (SBE) instacron:SBE |
| ISSN: | 1806-9665 |
| DOI: | 10.1590/1806-9665-rbent-2020-0080 |
| Popis: | Soybean looper (Chrysodeixis includens) and Spodoptera group are important defoliators insects responsible for significant yield losses in soybean, cotton and maize in Brazil. Bioinsecticides and transgenic plants expressing insecticidal proteins from Bacillus thuringiensis are among the most used sustainable control methods for pest control in agriculture, especially for several lepidopteran species. To provide new components for insect control, this study aimed to select and characterize B. thuringiensis strains toxic to C. includens, S. cosmioides, S. eridania, and S. frugiperda. We performed initial bioassays with fifty B. thuringiensis strains for C. includens and selected four strains – 1608A, 726, 773, and 775E – that caused high larval mortality (100%) to be tested against Spodoptera species. These strains harbored cry insecticidal genes, megaplasmids, absence of β-exotoxin and showed two major proteins about 65 and 130 kDa in SDS-page attributed to Cry protein classes toxic to lepidopteran species. The 1608A and 775E strains also showed high toxicity to Spodoptera species that demonstrate great potential for B. thuringiensis-based bioproduct development and as sources of new insecticidal genes for transgenic crops for multiple pest control relevant in the Brazilian agricultural system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|