Unregulated Expression of the Erythropoietin Receptor Gene Caused by Insertion of Spleen Focus-Forming Virus Long Terminal Repeat in a Murine Erythroleukemia Cell Line
| Autor: | Masabumi Shibuya, Akihiro Tojo, H Morii, F Takaku, Y Misawa, Masayuki Hino |
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| Rok vydání: | 1991 |
| Předmět: |
Gene Expression Regulation
Viral Molecular Sequence Data Restriction Mapping Oligonucleotides Receptors Cell Surface Biology Polymerase Chain Reaction Cell Line Mice Cell surface receptor Complementary DNA hemic and lymphatic diseases Gene expression Murine leukemia virus Receptors Erythropoietin Animals Amino Acid Sequence Cloning Molecular Erythropoietin Spleen Focus-Forming Viruses Gene Molecular Biology Repetitive Sequences Nucleic Acid Base Sequence food and beverages DNA Neoplasm Cell Biology biology.organism_classification Molecular biology Long terminal repeat Erythropoietin receptor Kinetics Mutagenesis Insertional Cell culture embryonic structures Leukemia Erythroblastic Acute Research Article |
| Zdroj: | Molecular and Cellular Biology. 11:5527-5533 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.11.11.5527-5533.1991 |
| Popis: | A murine erythroleukemia (MEL) cell line, F5-5, expressed 10,000 binding sites for erythropoietin (EPO) per cell, 10-fold more than was expressed by other murine erythroleukemia cell lines and normal erythroid progenitors. Northern (RNA) and Southern blot analyses revealed overexpression of mRNA for the EPO receptor (EPOR) and rearrangement of one of the EPOR gene alleles in F5-5 cells, respectively. Molecular cloning of F5-5-derived cDNA encoding EPOR revealed that the 5' noncoding region of the EPOR cDNA corresponds to the 3' long terminal repeat sequence of the polycythemic strain of Friend spleen focus-forming virus (F-SFFVP). The aberrant EPOR transcripts containing the 3' long terminal repeat sequence were mainly expressed in F5-5 cells. The same integration upstream of the EPOR gene was also observed in other subclones and the parent cell line. It is possible that overexpression of EPOR by viral promoter insertion will confer growth advantage to an F-SFFVP-infected erythroid progenitor cell, leading to positive clonal selection through further leukemogenic steps. |
| Databáze: | OpenAIRE |
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