Serotonin differentially modulates excitatory and inhibitory synaptic inputs to putative sleep-promoting neurons of the ventrolateral preoptic nucleus

Autor: Armelle Rancillac, Hélène Geoffroy, Aude Sangare, Romain Dubourget, Thierry Gallopin
Přispěvatelé: Laboratoire Plasticité du Cerveau Brain Plasticity (UMR 8249) (PdC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the French National Center for Scientific Research (CNRS), ESPCIParis Tech, and the French Institute of Health and Medical Research (Inserm).
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Single-cell RT-PCR
VLPO
[SDV]Life Sciences [q-bio]
Synaptic Transmission
EPSCs
chemistry.chemical_compound
Mice
0302 clinical medicine
kynurinic acid (PubChem CID: 3845)
Neurotransmitter
IPSCs
PF03246799 hydrochloride (PubChem CID 67097843)
noradrenaline (PubChem CID: 297812)
medicine.anatomical_structure
Editorial
Excitatory postsynaptic potential
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
non-rapid eye movement sleep (NREM)
Serotonin
Gal-GFP neurons
serotonin (PubChem CID: 160436)
NREM
Mice
Transgenic
slow-wave sleep (SWS)
Biology
Inhibitory postsynaptic potential
Serotonergic
03 medical and health sciences
Cellular and Molecular Neuroscience
Organ Culture Techniques
Cellular neuroscience
medicine
Animals
8-OH-DPAT (PubChem CID: 6917794)
5-HT1A receptor
5-HT receptor
Pharmacology
5-HT2C receptor
Excitatory Postsynaptic Potentials
Preoptic Area
Mice
Inbred C57BL
Electrophysiology
030104 developmental biology
chemistry
Inhibitory Postsynaptic Potentials
nervous system
Receptors
Serotonin
bicuculline (PubChem CID: 104871)
tetrodotoxin (PubChem CID: 16759596)
Soma
Sleep
Neuroscience
030217 neurology & neurosurgery
Zdroj: Neuropharmacology
Neuropharmacology, Elsevier, 2016, 109 (1), pp.29-40. ⟨10.1016/j.neuropharm.2016.05.015⟩
Oncotarget
ISSN: 0028-3908
Popis: International audience; The role of serotonin (5-HT) in sleep-wake regulation has been a subject of intense debate and remains incompletely understood. In the ventrolateral preoptic nucleus (VLPO), the main structure that triggers non-rapid eye movement (NREM) sleep, putative sleep-promoting (PSP) neurons were shown ex vivo to be either inhibited (Type-1) or excited (Type-2) by 5-HT application. To determine the complex action of this neurotransmitter on PSP neurons, we recorded spontaneous and miniature excitatory and inhibitory postsynaptic currents (sEPSCs, sIPSCs, mEPSCs and mIPSCs) in response to bath application of 5-HT. We established in mouse acute VLPO slices that 5-HT reduces spontaneous and miniature EPSC and IPSC frequencies to Type-1 neurons, whereas 5-HT selectively increases sIPSC and mIPSC frequencies to Type-2 VLPO neurons. We further determined that Type-1 neurons display a lower action potential threshold and a smaller soma size than Type-2 neurons. Finally, single-cell RT-PCR designed to identify the 13 serotonergic receptor subtypes revealed the specific mRNA expression of the 5-HT1A,B,D,F receptors by Type-1 neurons. Furthermore, the 5-HT2A-C,4,7 receptors were found to be equivalently expressed by both neuronal types. Altogether, our results establish that the excitatory and inhibitory inputs to Type-1 and Type-2 VLPO PSP neurons are differentially regulated by 5-HT. Electrophysiological, morphological and molecular differences were also identified between these two neuronal types. Our results provide new insights regarding the orchestration of sleep regulation by 5-HT release, and strongly suggest that Type-2 neurons could play a permissive role, whereas Type-1 neurons could have an executive role in sleep induction and maintenance.
Databáze: OpenAIRE
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