Effect of Graphene Family Materials on Multiple Myeloma and Non-Hodgkin’s Lymphoma Cell Lines
| Autor: | Norma C. Gutiérrez, Barbara Strojny, Piotr Koczoń, Marta Grodzik, Irena Misiewicz-Krzeminska, Isabel M. Isidro, Sławomir Jaworski, Ewa Sawosz, André Chwalibog, Elizabeta A. Rojas |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cancer cells
Lymphoma Non-adherent cells Cell lymphoma 02 engineering and technology 010402 general chemistry Cell morphology 01 natural sciences lcsh:Technology Article Flow cytometry law.invention law Multiple myeloma nanostructures medicine Journal Article General Materials Science Cytotoxicity lcsh:Microscopy Graphene oxide lcsh:QC120-168.85 non-adherent cells medicine.diagnostic_test lcsh:QH201-278.5 Graphene Chemistry lcsh:T graphene Cell cycle 021001 nanoscience & nanotechnology 0104 chemical sciences Nanostructures multiple myeloma medicine.anatomical_structure Cell culture lcsh:TA1-2040 Cancer cell Biophysics cancer cells graphene oxide lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 |
| Zdroj: | Strojny, B, Jaworski, S, Misiewicz-Krzeminska, I, Isidro, I, Rojas, E A, Gutiérrez, N C, Grodzik, M, Koczoń, P, Chwalibog, A & Sawosz, E 2020, ' Effect of Graphene Family Materials on Multiple Myeloma and Non-Hodgkin’s Lymphoma Cell Lines ', Materials, vol. 13, no. 15, 3420 . https://doi.org/10.3390/ma13153420 Materials, Vol 13, Iss 3420, p 3420 (2020) Strojny, B, Jaworski, S, Misiewicz-Krzemińska, I, Isidro, I, Rojas, E A, Gutiérrez, N C, Grodzik, M, Koczoń, P, Chwalibog, A & Sawosz, E 2020, ' Effect of graphene family materials on multiple myeloma and non-Hodgkin's lymphoma cell lines ', Materials, vol. 13, no. 15, 3420 . https://doi.org/10.3390/ma13153420 Materials Volume 13 Issue 15 |
| DOI: | 10.3390/ma13153420 |
| Popis: | The interest around the graphene family of materials is constantly growing due to their potential application in biomedical fields. The effect of graphene and its derivatives on cells varies amongst studies depending on the cell and tissue type. Since the toxicity against non-adherent cell lines has barely been studied, we investigated the effect of graphene and two different graphene oxides against four multiple myeloma cell lines, namely KMS-12-BM, H929, U226, and MM.1S, as well as two non-Hodgkin lymphoma cells lines, namely KARPAS299 and DOHH-2. We performed two types of viability assays, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide conversion) and ATP (adenosine triphosphate detection), flow cytometry analysis of apoptosis induction and cell cycle, cell morphology, and direct interaction analysis using two approaches&mdash visualization of living cells by two different systems, and visualization of fixed and dyed cells. Our results revealed that graphene and graphene oxides exhibit low to moderate cytotoxicity against cells, despite visible interaction between the cells and graphene oxide. This creates possibilities for the application of the selected graphene materials for drug delivery systems or theragnostics in hematological malignancies however, further detailed studies are necessary to explain the nature of interactions between the cells and the materials. |
| Databáze: | OpenAIRE |
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