Attenuated Salmonella typhimurium invades and decreases tumor burden in neuroblastoma
| Autor: | Arnold S. Leonard, Daniel A. Saltzman, Sean J. Barnett, Leland J. Soto, Brent W. Nelson, Brent S. Sorenson |
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| Rok vydání: | 2005 |
| Předmět: |
Salmonella typhimurium
Interleukin 2 Salmonella Mice Inbred A medicine.medical_treatment Green Fluorescent Proteins Biology medicine.disease_cause Microbiology Green fluorescent protein Mice Neuroblastoma In vivo Tumor Cells Cultured medicine Animals Retroperitoneal Neoplasms Electroporation General Medicine Immunotherapy medicine.disease Molecular biology In vitro Tumor Burden Disease Models Animal Pediatrics Perinatology and Child Health Interleukin-2 Female Surgery medicine.drug |
| Zdroj: | Journal of Pediatric Surgery. 40:993-998 |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/j.jpedsurg.2005.03.015 |
| Popis: | Introduction We have previously shown that Salmonella elicits an antitumor response against hepatic adenocarcinomatous metastases. In vitro studies have demonstrated both intracellular invasion and proliferation of Salmonella within cultured neuroblastoma cells. We sought to demonstrate in vivo invasion, proliferation, and a potential antitumor response. Methods A murine model for retroperitoneal neuroblastoma was established with viable neuroblastoma cells. A green fluorescent protein (GFP—Clontech, Palo Alto, CA) gene was inserted into our attenuated Salmonella species via electroporation. Fourteen days after retroperitoneal injection, the Salmonella typhimurium - pGFP construct was administered and studied. In separate experiments, the antitumor effect against neuroblastoma was studied in controls, Salmonella lacking an interleukin 2 (IL-2) gene (Sal-NG), and Salmonella containing an IL-2 gene ( Salmonella-pIL2 ). Results Consistent with previous reports, 74% of mice injected were found to have recognizable tumors. Salmonella was present within tumor cells. Average tumor volumes for control, Sal-NG, and Salmonella-pIL2 mice were 2024.3, 749.5, and 332.4 mm 3 , respectively ( P Salmonella-pIL2 mice were 2.218, 0.880, and 0.377 g, respectively ( P Conclusions Attenuated Salmonella species can now be tracked via fluorescent microscopy within tumor cells. Furthermore, an 84% reduction in tumor burden was observed in those animals gavage fed Salmonella with the IL-2 gene. |
| Databáze: | OpenAIRE |
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