A Healthy Carrier of Verotoxin-Producing Escherichia coli, Who Was Detected by Periodic Feces Examinations
| Autor: | Tae Takeda, Wakio Minamide, Kiyohisa Uchida, Hirotune Igimi, Yutaka Murata, Shinji Kusunoki |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
Male Bacterial Toxins Biology Shiga Toxin 1 medicine.disease_cause Polymerase Chain Reaction Rapid detection Microbiology Feces Escherichia coli medicine Humans Child Escherichia coli Infections Aged General Medicine Verotoxin-Producing Escherichia coli Middle Aged Diarrhea Antibiotic Agents VTEC Carrier State Female medicine.symptom Asymptomatic carrier |
| Zdroj: | Journal of the Japanese Association for Infectious Diseases. 67:1211-1218 |
| ISSN: | 1884-569X 0387-5911 |
| Popis: | Verotoxin-producing Escherichia coli (VTEC) was rapidly detected by the PCR method in one of the 9 feces samples. They were collected from the people who had been cooking meals for patients on a periodic feces examination on November 25, 1992. It was confirmed by PCR that the isolate had both VT1 and VT2vh toxic genes and showed cytotoxicity on Verocells. In this case, the isolate was highly susceptible to common antibiotic agents, it was removed by the administration of tosfulaxacin. Some isolates which had the same properties as those of the strain described in "VITEK GNI card" and the antimicrobial susceptibility and VT toxin types, were detected in 2 of the 3 members of her family, they were healthy carriers and did not have any subjective symptoms such as diarrhea or abdominal pain. In order to detect Verotoxic genes, the sample of feces preincubated for 3 h in trypticase broth was subjected to PCR. We recommend that this method is much more useful because of rapid detection and identification of VTEC compared with the classical culture method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|