Transcriptional Repression by RING Finger Protein TIF1 That Interacts with the KRAB Repressor Domain of KOX1
| Autor: | Peter Moosmann, Oleg Georgiev, Walter Schaffner, B Le Douarin, Jean-Pierre Bourquin |
|---|---|
| Přispěvatelé: | University of Zurich, Schaffner, W |
| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Molecular Sequence Data 610 Medicine & health Tripartite Motif-Containing Protein 28 Biology 142-005 142-005 1311 Genetics Krüppel Genetics Ring finger medicine Humans Amino Acid Sequence Cloning Molecular In Situ Hybridization Fluorescence LIM domain Zinc finger Sequence Homology Amino Acid C2H2 Zinc Finger Chromosome Mapping Zinc finger nuclease Molecular biology Recombinant Proteins DNA-Binding Proteins Repressor Proteins RING finger domain medicine.anatomical_structure PHD finger 570 Life sciences biology Chromosomes Human Pair 19 HeLa Cells Research Article |
| Zdroj: | Nucleic Acids Research. 24:4859-4867 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/24.24.4859 |
| Popis: | Many of the vertebrate zinc finger factors of the Kruppel type (C2H2 zinc fingers) contain in their N-terminus a conserved sequence referred to as the KRAB (Kruppel-associated box) domain that, when tethered to DNA, efficiently represses transcription. Using the yeast two-hybrid system, we have isolated an 835 amino acid RING finger (C3HC4 zinc finger) protein, TIF1 beta (also named KAP-1), that specifically interacts with the KRAB domain of the human zinc finger factor KOX1/ZNF10. TIF1 beta, TIF1 alpha, PML and efp belong to a characteristic subgroup of RING finger proteins that contain one or two other Cys/His-rich clusters (B boxes) and a putative coiled-coil in addition to the classical C3HC4 RING finger motif (RBCC configuration). Like TIF1 alpha, TIF1 beta also contains an additional Cys/His cluster (PHD finger) and a bromo-related domain. When tethered to DNA, TIF1 beta can repress transcription in transiently transfected mammalian cells both from promoter-proximal and remote (enhancer) positions, similarly to the KRAB domain itself. We propose that TIF1 beta is a mediator of the transcriptional repression exerted by the KRAB domain. |
| Databáze: | OpenAIRE |
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