Molecular characterisation of Beauveria bassiana isolates obtained from overwintering sites of Sunn Pests (Eurygaster and Aelia species)
| Autor: | Marilena Aquino de Muro, David Moore, Sarah Elliott, Margaret Skinner, Bruce L. Parker, Mustapha El Bouhssini, William Reid |
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| Rok vydání: | 2005 |
| Předmět: |
Genetic diversity
fungi food and beverages Beauveria bassiana Plant Science Bassiana Biology biology.organism_classification Polymerase Chain Reaction Sunn pest Heteroptera Ascomycota Genetic variation Botany Genetics Animals Eurygaster Beauveria DNA Fungal Ecology Evolution Behavior and Systematics Overwintering Polymorphism Restriction Fragment Length Biotechnology |
| Zdroj: | Mycological research. 109(Pt 3) |
| ISSN: | 0953-7562 |
| Popis: | 110 isolates of Beauveria (104 B. bassiana, 5 Beauveria spp., 1 B. brongniartii) were obtained from Sunn Pests (Eurygaster and Aelia species), litter, and other insect samples at overwintering sites in seven countries in the Middle East and West Asia. DNA was extracted from these isolates, and four techniques were used to characterize and to investigate genetic diversity at the molecular level: ITS-RFLP, ITS sequencing, ISSR-PCR, and AFLP. The ITS-RFLP and ITS sequences did not detect significant genetic variation among the isolates. However, both ISSR-PCR and AFLP analyses gave indications of intraspecific groupings correlated with geographical origin and relative genetic diversity among some isolates, but no obvious association with Sunn Pest hosts. There was no obvious genotypic grouping of B. bassiana isolates from E. integriceps, perhaps suggesting the overwintering populations were infected by generalist native isolates rather than by host-specific ones that might be more suitable for biocontrol purposes. |
| Databáze: | OpenAIRE |
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