Langerhans cell histiocytosis and human herpes virus 6 (HHV-6), an analysis by real-time polymerase chain reaction
| Autor: | Michael P. Glotzbecker, Yagnya Joshi, John P. Dormans, Richard L. Hodinka, Brian P.D. Wills, Michael Elkan, Bruce R. Pawel |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent viruses Herpesvirus 6 Human Population Disease Biology Pathogenesis Langerhans cell histiocytosis TaqMan medicine Humans Orthopedics and Sports Medicine education Child education.field_of_study Reverse Transcriptase Polymerase Chain Reaction Infant Herpesviridae Infections Viral Load medicine.disease Histiocytosis Histiocytosis Langerhans-Cell Real-time polymerase chain reaction Child Preschool Immunology DNA Viral Female Viral load |
| Zdroj: | Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 24(3) |
| ISSN: | 0736-0266 |
| Popis: | Patients with Langerhans cell histiocytosis (LCH) usually present to orthopedic surgeons because this disease most commonly affects bone. The pathogenesis of LCH is unknown, although roles for environmental, infectious, immunologic, and genetic causes have been postulated. More specifically, there is limited data suggesting that human herpes virus 6 (HHV-6) may be a potential etiologic agent. Frozen biopsy material was obtained from 13 patients with LCH and 20 patients without the disease. After ensuring histologic adequacy of the material, the tissue was tested for HHV-6 by qualitative and quantitative real-time TaqMan PCR. Four of 13 patients with LCH had evidence of HHV-6 DNA in their tissue while 7 of 20 control patients tested positive for HHV-6 genome. Viral loads are reported for the positive patients; no statistical difference was observed in the presence or quantity of HHV-6 DNA found in either population, suggesting that the prevalence of HHV-6 in the tissue of LCH patients is the same as that found in tissue from individuals without disease. |
| Databáze: | OpenAIRE |
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