Down-regulation of β3-adrenergic receptor expression in rat adipose tissue during the fasted/fed transition: evidence for a role of insulin
| Autor: | Jacques Pairault, Annie Quignard-Boulangé, Sylvie Hauguel-de Mouzon, Christine Charon, Khadija El Hadri, Bruno Fève |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Adrenergic receptor medicine.medical_treatment Adipose tissue White adipose tissue Biology Biochemistry Propanolamines Eating Mice Adipose Tissue Brown stomatognathic system Downregulation and upregulation Internal medicine Receptors Adrenergic beta Brown adipose tissue Adipocytes medicine Animals Insulin RNA Messenger Rats Wistar Molecular Biology 3T3 Cells Fasting Cell Biology Adrenergic beta-Agonists Rats Endocrinology medicine.anatomical_structure Ethanolamines Receptors Adrenergic beta-3 Adenylyl Cyclase Inhibitors Catecholamine Cyclase activity Research Article medicine.drug |
| Zdroj: | Biochemical Journal. 323:359-364 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3230359 |
| Popis: | The beta3-adrenergic receptor (beta3-AR) exerts a central role in the transduction of catecholamine effects in white and brown adipose tissue (WAT and BAT). A recent report has documented that insulin strongly down-regulates beta3-AR expression and catecholamine responsiveness in 3T3-F442A adipocytes [Fève, El Hadri, Quignard-Boulangé and Pairault (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 5677-5681]. In the present report we show that the rise in plasma insulin levels elicited by the fasted/fed transition is associated with a reduction in beta3-AR mRNA levels and beta-adrenergic responsiveness in WAT and BAT. beta3-AR transcripts are also decreased in adipose tissue from animals subjected for 6 h to euglycaemic hyperinsulinaemic glucose clamps. Moreover, insulin acts directly on cultured rat white and brown adipocytes to decrease beta3-AR gene expression and adenylate cyclase activity in response to beta3-AR-selective agonists. These results suggest that there is a close relationship between food intake, plasma insulin levels and beta3-AR expression. |
| Databáze: | OpenAIRE |
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