Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood
| Autor: | Zuzanna Kaźmierczak, Agnieszka Piotrowicz, Barbara Owczarek, Krystyna Dąbrowska, Joanna Majewska, Jarosław Ciekot, Marek Harhala, Weronika Beta, Paulina Miernikiewicz, Agnieszka Kopciuch, Dorota Lecion, Karolina Wojtyna, Mateusz Mąkosa, Anna Kłopot, Katarzyna Hodyra-Stefaniak |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Immunoglobulin A
Male Phage display viruses lcsh:QR1-502 Administration Oral medicine.disease_cause Antibodies Viral Increased IgA level lcsh:Microbiology Immunoglobulin G Article capsid proteins T4 phage EBOV Ebola virus oral administration vaccine phage display antibodies phage resistance Microbiology Antigen Virology medicine Escherichia coli Animals Bacteriophage T4 Longitudinal Studies Antigens Viral Immunity Mucosal Viral Structural Proteins biology Ebolavirus Gastrointestinal Tract Mice Inbred C57BL Infectious Diseases Blood Immunoglobulin M biology.protein Antibody |
| Zdroj: | Viruses Viruses, Vol 7, Iss 8, Pp 4783-4799 (2015) Viruses; Volume 7; Issue 8; Pages: 4783-4799 Volume 7 Issue 8 Pages 4783-4799 |
| ISSN: | 1999-4915 |
| Popis: | A specific humoral response to bacteriophages may follow phage application for medical purposes, and it may further determine the success or failure of the approach itself. We present a long-term study of antibody induction in mice by T4 phage applied per os: 100 days of phage treatment followed by 112 days without the phage, and subsequent second application of phage up to day 240. Serum and gut antibodies (IgM, IgG, secretory IgA) were analyzed in relation to microbiological status of the animals. T4 phage applied orally induced anti-phage antibodies when the exposure was long enough (IgG day 36, IgA day 79) the effect was related to high dosage. Termination of phage treatment resulted in a decrease of IgA again to insignificant levels. Second administration of phage induces secretory IgA sooner than that induced by the first administrations. Increased IgA level antagonized gut transit of active phage. Phage resistant E. coli dominated gut flora very late, on day 92. Thus, the immunological response emerges as a major factor determining phage survival in the gut. Phage proteins Hoc and gp12 were identified as highly immunogenic. A low response to exemplary foreign antigens (from Ebola virus) presented on Hoc was observed, which suggests that phage platforms can be used in oral vaccine design. |
| Databáze: | OpenAIRE |
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