Schistosoma mansoni: phenol oxidase's role in eggshell formation
| Autor: | James L. Bennett, John L. Seed |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Protein polymerization
Immunology Eggshell formation Ascorbic Acid Lipid peroxidation chemistry.chemical_compound Egg Shell Mice parasitic diseases Animals Vitamin E Eggshell Oxidase test biology Autoxidation General Medicine Schistosoma mansoni biology.organism_classification Infectious Diseases chemistry Biochemistry Peroxidases biology.protein Tyrosine Parasitology Female Ditiocarb Catechol Oxidase Peroxidase Phenylthiazolylthiourea |
| Zdroj: | Experimental parasitology. 49(3) |
| ISSN: | 0014-4894 |
| Popis: | Phenol oxidase may be involved in the formation of the eggshell in Schistosoma mansoni. 3H-Labeled female S. mansoni proteins were polymerized in vitro following incubation with S. mansoni phenol oxidase and excess l -tyrosine. Peroxidase inhibitors, autoxidation inhibitors, inhibitors of lipid peroxidation, and inactive analogs of phenol oxidase inhibitors did not inhibit eggshell formation. Fluorescent substances found in eggshell hydrolysates were similar to those formed from the reaction of phenol oxidase-generated quinones with protein-bound lysine. These observations support the classical concept of phenol oxidase-catalyzed protein hardening. However, fluorescent globules of egg material were still formed after the administration of 200 mg/kg of the phenol oxidase inhibitor diethyldithiocarbamate. These globules could not be destroyed by inhibitors of autoxidation and lipid peroxidation. |
| Databáze: | OpenAIRE |
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