Combination of bone morphogenetic protein-2 plasmid DNA with chemokine CXCL12 creates an additive effect on bone formation onset and volume
| Autor: | F. C. Oner, Fiona Wegman, Michelle T. Poldervaart, Jacqueline Alblas, Y. J. van der Helm, Wouter J.A. Dhert |
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| Rok vydání: | 2015 |
| Předmět: |
Bone Regeneration
Stromal cell Bone morphogenetic protein 8A Biomedical Engineering Bone Morphogenetic Protein 2 Bioengineering Biochemistry Bone morphogenetic protein 2 Multipotent stromal cells Biomaterials Mice Gene therapy Tissue engineering Controlled release Animals Animal model Bone Bone regeneration Cells Cultured Chemistry Goats Cell Differentiation Mesenchymal Stem Cells Cell Biology DNA Chemokine CXCL12 Stromal cell-derived factor-1α Cell biology Bone morphogenetic protein 7 Bone morphogenetic protein 6 Bone morphogenetic protein 5 Immunology Bone morphogenetic protein-2 Plasmids |
| Zdroj: | European Cells & Materials, 30, 1. Swiss Society for Biomaterials European Cells & Materials, 30 |
| ISSN: | 1473-2262 |
| DOI: | 10.22203/ecm.v030a01 |
| Popis: | Bone morphogenetic protein-2 (BMP-2) gene delivery has shown to induce bone formation in vivo in cell-based tissue engineering. In addition, the chemoattractant stromal cell-derived factor-1α (SDF-1α, also known as CXCL12) is known to recruit multipotent stromal cells towards its release site where it enhances vascularisation and possibly contributes to osteogenic differentiation. To investigate potential cooperative behaviour for bone formation, we investigated combined release of BMP-2 and SDF-1α on ectopic bone formation in mice. Multipotent stromal cellseeded and cell-free constructs with BMP-2 plasmid DNA and /or SDF-1α loaded onto gelatin microparticles, were implanted subcutaneously in mice for a period of 6 weeks. Histological analysis and histomorphometry revealed that the onset of bone formation and the formed bone volume were both enhanced by the combination of BMP-2 and SDF-1α compared to controls in cell-seeded constructs. Samples without seeded multipotent stromal cells failed to induce any bone formation. We conclude that the addition of stromal cellderived factor-1α to a cell-seeded alginate based bone morphogenetic protein-2 plasmid DNA construct has an additive effect on bone formation and can be considered a promising combination for bone regeneration. |
| Databáze: | OpenAIRE |
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