Quantitation of sulfhydryl groups on erythrocytes in polycythemia vera
| Autor: | M. W. Hougland, J. M. Luethke |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Histology
Erythrocytes chemistry.chemical_compound Polycythemia vera hemic and lymphatic diseases medicine Humans Sulfhydryl Compounds Molecular Biology Polycythemia Vera chemistry.chemical_classification Mixed disulfides Chromatography Erythrocyte Membrane Cell Biology General Medicine Glutathione medicine.disease Control subjects Medical Laboratory Technology Biochemistry chemistry Reagent Thiol Anatomy General Agricultural and Biological Sciences |
| Zdroj: | Histochemistry. 76(1) |
| ISSN: | 0301-5564 |
| Popis: | The number of sulfhydryl groups on the surface of intact erythrocytes from patients with polycythemia vera is significantly less (34%) than those from control subjects. Sulfhydryl groups were measured indirectly by reversibly blocking -SH groups with the thiol reagent, 6,6'-dithiodinicotinic acid, which forms stable mixed disulfides on the surface of erythrocytes. Glutathione was used to break the disulfides and release thione into the supernatant. Thione was then quantitated spectrophotometrically. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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