Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae
| Autor: | Tadashi Wada, Hiroshi Handa, Fred Winston, Grant A. Hartzog |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Transcription Genetic Chromosomal Proteins Non-Histone Genes Fungal Molecular Sequence Data RNA polymerase II Saccharomyces cerevisiae Biology Fungal Proteins Transcription Elongation Factor SPT5 Genetics Histone Chaperones Amino Acid Sequence Negative elongation factor Genes Suppressor RNA polymerase II holoenzyme Sequence Homology Amino Acid General transcription factor Nuclear Proteins Molecular biology Cold Temperature Mutation biology.protein Transcription factor II F RNA Polymerase II Transcription factor II E Transcription Factors General Transcriptional Elongation Factors Transcription factor II D Research Paper Protein Binding Transcription Factors Developmental Biology |
| Zdroj: | Genes & Development. 12:357-369 |
| ISSN: | 0890-9369 |
| DOI: | 10.1101/gad.12.3.357 |
| Popis: | Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we report new genetic and biochemical studies of Spt4, Spt5, and Spt6 that reveal a role for these factors in transcription elongation. We have isolated conditional mutations in SPT5 that can be suppressed in an allele-specific manner by mutations in the two largest subunits of RNA polymerase II (Pol II). Strikingly, one of these RNA Pol II mutants is defective for transcription elongation and the others cause phenotypes consistent with an elongation defect. In addition, we show that spt4, spt5, and spt6 mutants themselves have phenotypes suggesting defects in transcription elongation in vivo. Consistent with these findings, we show that Spt5 is physically associated with RNA Pol II in vivo, and have identified a region of sequence similarity between Spt5 and NusG, an Escherichia coli transcription elongation factor that binds directly to RNA polymerase. Finally, we show that Spt4 and Spt5 are tightly associated in a complex that does not contain Spt6. These results, taken together with the biochemical identification of a human Spt4-Spt5 complex as a transcription elongation factor (Wada et al. 1998), provide strong evidence that these factors are important for transcription elongation in vivo. |
| Databáze: | OpenAIRE |
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