Molecular epidemiology of entamoeba : First description of Entamoeba moshkovskii in a rural area from central Colombia
| Autor: | Ligia I. Moncada, Patricia Reyes, Juan David Ramírez, Cielo León, Myriam Consuelo López, Jairo Andres Fonseca, Mario Javier Olivera |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Rural Population
Male lcsh:Medicine Polymerase Chain Reaction Entamoeba Feces fluids and secretions Infección asintomática Infección amebiana Microscopía Reacción en cadena de la polimerasa Prevalence Entamoeba moshkovskii Asymptomatic Infection Parasitología lcsh:Science Masculino Preschool Child Child Extracción de ADN Adolescente Phylogeny Molecular Epidemiology Microscopy Multidisciplinary Infecciones biology Entamoebiasis Población rural Coinfection Rna 18S Feces analysis Amebiasis Amebic Infection Classification Child Preschool Niño Female Entamoeba Dispar Niño preescolar ARN 18C Research Article Heces Incidencia & prevención de la enfermedad Human Adult Dna Sequence Adolescent Rural Area Dispar Major Clinical Study Clasificación Colombia Secuencia de ADN Microbiology Infección por Entamoeba muscovensis Entamoeba histolytica Recién nacido Estudio clínico principal Dna Extraction parasitic diseases RNA Ribosomal 18S Genetics Humans Epidemiología Entamoeba Dispar Infection Análisis de heces Infección mixta Humano Molecular epidemiology lcsh:R Entamoeba Moshkovskii Infection Infant Newborn Infant Mixed Infection biology.organism_classification Newborn Genética Estudio transversal Área rural Cross-Sectional Study Filogenia Parasitology lcsh:Q Coinfección Predominio Epidemiología Molecular Feces Analysis |
| Zdroj: | WHO/PAHO/UNESCO report of a consultation with experts on amoebiasis (1997) Epidemiol Bull., 18, pp. 13-14 Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario PLoS ONE PLoS ONE, Vol 10, Iss 10, p e0140302 (2015) |
| Popis: | Background Entamoeba histolytica, E. dispar and E. moshkovskii are the most frequent species described in human infection where E. histolytica is the only true pathogen. The epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the Entamoeba species complex. Therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. Herein, we conducted a cross-sectional study to determine the frequency of Entamoeba species infections in a group of asymptomatic individuals from a rural area in central Colombia. Methodology/Principal Findings A total of 181 fecal samples from asymptomatic children under 16 years old from the hamlet La Vírgen, Cundinamarca (Colombia) that voluntarily accepted to participate in the study were collected. The fecal samples were examined by light microscopy and DNA-extracted, subsequently submitted to molecular discrimination of E. dispar/E. histolytica/E. moshkovskii infection based on a multiplex PCR assay targeting the 18S rRNA fragment. To confirm the species description, twenty samples were randomly submitted to DNA sequencing of the aforementioned fragment. By direct microscopic examination, frequency of the complex E. histolytica/E. dispar/E. moshkovskii was 18.8%(34/181). PCR showed a frequency of 49.1% (89/181), discriminated as 23.2%(42/181) that were positive for E. dispar, 25.4% (46/181) for E. moshkovskii and 0.55% (1/ 181) for E. histolytica. Also, mixed infections were detected between E. dispar and E. moshkovskii at 4.42% (8/181) of the samples. Molecular barcoding confirmed the diagnosis depicted by the multiplex PCR assay. Conclusions/Significance This is the first description of E. moshkovskii in Colombia and the second report in South- America to our knowledge. Our results suggest the need to unravel the true epidemiology of Entamoeba infections around the world, including the real pathogenic role that E. moshkovskii may have. Copyright: © 2015 López et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|