Chromosomal changes in cell lines from mouse tumors induced by nickel sulfide and methylcholanthrene
| Autor: | Donna M. Tummolo, Neal W. Biggart, Nupelwyn T. Christie, E C Murphy |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Nickel sulfide
Carcinogenicity Tests Health Toxicology and Mutagenesis Marker chromosome chemistry.chemical_element Aneuploidy Toxicology Mice Chromosome 15 chemistry.chemical_compound Nickel Rhabdomyosarcoma Tumor Cells Cultured medicine Animals Chromosome Aberrations Karyotype Cell Biology Blotting Northern medicine.disease Molecular biology Chromosome Banding Blotting Southern Chromosome 4 chemistry Cell culture Karyotyping Female Methylcholanthrene |
| Zdroj: | Cell Biology and Toxicology. 4:427-445 |
| ISSN: | 1573-6822 0742-2091 |
| Popis: | Rhabdomyosarcomas were induced in mice by intramuscular injections of crystalline nickel sulfide and 3-methylcholanthrene. At early passage, karyotypes were performed by G-banding for four nickel sulfide cell lines and for three 3-methylcholanthrene cell lines. Six cell lines were near-diploid and one nickel sulfide line was near-tetraploid. Three of the nickel sulfide cell lines were characterized by a rearranged marker chromosome which was present in a majority of the cells of each line. The rearrangements leading to the formation of marker chromosomes were different in each nickel sulfide cell line but involved chromosome 4 in two of the nickel sulfide cell lines. Extra copies of chromosome 15 were present in two nickel sulfide cell lines. Possible rearrangement and/or gene activation was examined for the c-mos oncogene on chromosome 4 and the c-myc oncogene on chromosome 15, but no alteration or activation was observed. None of the 3-methylcholanthrene cell lines contained rearranged marker chromosomes; however, one MCA cell line did contain large numbers of double minutes. In all cell lines, minichromosomes (small atypical acrocentric chromosomes) were observed that contained distinct centromeric regions but no other G-positive bands. |
| Databáze: | OpenAIRE |
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