pH Responsive Abelmoschus esculentus Mucilage and Administration of Methotrexate: In-Vitro Antitumor and In-Vivo Toxicity Evaluation
| Autor: | Sobia Noreen, Sara Hasan, Shazia Akram Ghumman, Syed Nasir Abbas Bukhari, Bushra Ijaz, Huma Hameed, Huma Iqbal, Afeefa Aslam, Mervat Abdelaziz Mohamed Elsherif, Shazia Noureen, Hasan Ejaz |
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| Přispěvatelé: | University of Sargodha, University of Health Sciences [Lahore] (UHS Lahore), Jouf University, University of the Punjab, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Organic Chemistry technology industry and agriculture General Medicine pH-responsiveness Catalysis Computer Science Applications Inorganic Chemistry biopolymer in-vivo toxicity anticancer drug antitumor activity sustained delivery Physical and Theoretical Chemistry Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2022, 23 (5), ⟨10.3390/ijms23052725⟩ International Journal of Molecular Sciences; Volume 23; Issue 5; Pages: 2725 |
| ISSN: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms23052725⟩ |
| Popis: | The rapid progression in biomaterial nanotechnology apprehends the potential of non-toxic and potent polysaccharide delivery modules to overcome oral chemotherapeutic challenges. The present study is aimed to design, fabricate and characterize polysaccharide nanoparticles for methotrexate (MTX) delivery. The nanoparticles (NPs) were prepared by Abelmoschus esculentus mucilage (AEM) and chitosan (CS) by the modified coacervation method, followed by ultra-sonification. The NPs showed much better pharmaceutical properties with a spherical shape and smooth surface of 213.4–254.2 nm with PDI ranging between 0.279–0.485 size with entrapment efficiency varying from 42.08 ± 1.2 to 72.23 ± 2.0. The results revealed NPs to possess positive zeta potential and a low polydispersity index (PDI). The in-vitro drug release showed a sustained release of the drug up to 32 h with pH-dependence. Blank AEM -CS NPs showed no in-vivo toxicity for a time duration of 14 days, accompanied by high cytotoxic effects of optimized MTX loaded NPs against MCF-7 and MD-MBA231 cells by MTT assay. In conclusion, the findings advocated the therapeutic potential of AEM/CS NPs as an efficacious tool, offering a new perspective for pH-responsive routing of anticancer drugs with tumor cells as a target. |
| Databáze: | OpenAIRE |
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