Covalent Attachment of Gold Nanoparticles to DNA Templates
| Autor: | Jack C. Wells, Karen A. Stevenson, Jacob Barhen, Govindarajan Muralidharan, Thomas Thundat, Leon Maya |
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| Rok vydání: | 2002 |
| Předmět: |
Materials science
Macromolecular Substances Surface Properties Biomedical Engineering Nanoparticle Bioengineering Microscopy Atomic Force chemistry.chemical_compound Nanotechnology General Materials Science Sulfhydryl Compounds Particle Size Nanodevice Electrophoresis Agar Gel Gel electrophoresis Binding Sites Staining and Labeling Thioctic Acid Spectrophotometry Atomic DNA Templates Genetic General Chemistry Condensed Matter Physics Combinatorial chemistry Microspheres Thymine chemistry Covalent bond Colloidal gold Gold Thymidine |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 2:397-404 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2002.110 |
| Popis: | Functionalized gold nanoparticles have been covalently bound to internal, modified sites on double-stranded DNA. Gold nanoparticles coated with mercaptosuccinic acid or thioctic acid were bound to amino-modified thymine bases on double-stranded DNA. Visible absorption spectra, gel electrophoresis, and atomic force microscopy were used to analyze the products. Thiol groups were added to one end of the gold/nanoparticle product, which was then attached to a gold surface. This method has the potential to allow controlled placement of particles with subnanometer precision and to allow attachment of the product to fixed contacts for nanodevice fabrication. |
| Databáze: | OpenAIRE |
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