Expression of Local Hepatocyte Growth Factor System in Vascular Tissues
| Autor: | Ryuichi Morishita, Atsushi Moriguchi, Toshio Ogihara, Kazuo Yamada, Motokuni Aoki, Yoshikage Yo, Shin-ichiro Hayashi, Y. Nakamura, Kunio Matsumoto, Jitsuo Higaki, Toshikazu Nakamura, Iwao Kida |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Endothelium medicine.medical_treatment Molecular Sequence Data Cell Biophysics Gene Expression Polymerase Chain Reaction Biochemistry Muscle Smooth Vascular Rats Sprague-Dawley Internal medicine medicine Animals Humans Aorta Abdominal RNA Messenger Rats Wistar Receptor Molecular Biology Aorta Vascular tissue DNA Primers Base Sequence Hepatocyte Growth Factor Chemistry Growth factor Receptor Protein-Tyrosine Kinases Cell Biology Proto-Oncogene Proteins c-met Coronary Vessels Rats Cell biology Vascular endothelial growth factor B Endocrinology medicine.anatomical_structure cardiovascular system Hepatocyte growth factor Endothelium Vascular Tunica Intima medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 215:483-488 |
| ISSN: | 0006-291X |
| Popis: | Since endothelial cells (EC) are known to secrete various anti-proliferative and vasodilating factors, an agent that promotes seeding or regeneration of EC may have potential therapeutic value against vascular smooth muscle cell (VSMC) proliferation. For the above purpose, we have found that hepatocyte growth factor (HGF) fulfills such conditions. However, the local HGF system has not yet clarified. Therefore, we investigated endogenous HGF production and its specific receptor (c-met) in vascular tissues. Our results revealed the expressions of HGF and its receptor (c-met) mRNA in endothelial cells, VSMC and neointimal VSMC of rat assessed by RT-PCR. HGF and c-met mRNA were also detected in human aortic endothelial cells and VSMC. Endothelial cells and VSMC of both rat and human produce and secrete immunoreactive HGF as assessed by ELISA. Of importance, the existence of local HGF system (HGF and c-met) was also observed in vivo in intact abdominal aorta of SD and Wistar rats. Overall, this study demonstrated that HGF is expressed and secreted from endothelial cells and VSMC of both rat and human in vitro as well as in vivo. Since HGF has characteristics of an endothelium-specific growth factor, locally synthesized HGF in endothelial cells and VSMC may have a role in vascular functions in autocrine-paracrine manner. |
| Databáze: | OpenAIRE |
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