Size-Based Chromatography of Signaling Clusters in a Living Cell Membrane
| Autor: | Geoff P. O’Donoghue, Yan Yu, Niña G. Caculitan, Eulanca Y. Liu, Nicole C. Fay, Hiroyuki Kai, Theobald Lohmüller, Jay T. Groves |
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| Rok vydání: | 2014 |
| Předmět: |
Letter
Materials science Receptors Antigen T-Cell Metal Nanoparticles Bioengineering Nanotechnology Living cell Cell membrane Nanodot array medicine General Materials Science Metal nanoparticles Chromatography Nanotubes Mechanical Engineering Cell Membrane Membrane Substrate (chemistry) cluster chromatography General Chemistry Condensed Matter Physics medicine.anatomical_structure Colloidal gold nanodot array Gold T cell receptor Signal Transduction |
| Zdroj: | Nano Letters |
| ISSN: | 1530-6992 1530-6984 |
| Popis: | Here we introduce a form of chromatography that can be imposed on the membrane of a living cell. A cell–cell signaling interaction is reconstituted in a hybrid live cell-supported membrane junction. The chromatographic material consists of a hexagonally ordered array of gold nanoparticles (nanodot array), which is fabricated onto the underlying substrate. While individual membrane components move freely throughout the array, the movement of larger assemblies is impeded if they exceed the physical dimensions of the array. This tactile approach to probing membrane structures in living cells reveals organizational aspects of the membrane environment unobservable by other techniques. |
| Databáze: | OpenAIRE |
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