Genome-Wide Identification and Functional Characterization of GATA Transcription Factor Gene Family in Alternaria alternata
| Autor: | Haijie Ma, Yanan Chen, Zengrong Zhu, Kuang-Ren Chung, Yingzi Cao, Yunpeng Gai, Hongye Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Genetics biology QH301-705.5 fungal development Conidiation Virulence Plant Science biology.organism_classification Alternaria alternata systematic analysis Article GATA transcription factor Transcriptome pathogenicity RNA-seq Gene family Biology (General) Gene Ecology Evolution Behavior and Systematics Gene knockout |
| Zdroj: | Journal of Fungi, Vol 7, Iss 1013, p 1013 (2021) Journal of Fungi Journal of Fungi; Volume 7; Issue 12; Pages: 1013 |
| Popis: | In the present study, we identified six GATA transcription factors (AaAreA, AaAreB, AaLreA, AaLreB, AaNsdD, and AaSreA) and characterized their functions in response to environmental stress and virulence in the tangerine pathotype of Alternaria alternata. The targeted gene knockout of each of the GATA-coding genes decreased the growth to varying degrees. The mutation of AaAreA, AaAreB, AaLreB, or AaNsdD decreased the conidiation. All the GATA transcription factors were found to be required for tolerance to cumyl hydroperoxide and tert-butyl-hydroperoxide (oxidants) and Congo red (a cell-wall-destructing agent). Pathogenicity assays assessed on detached citrus leaves revealed that mutations of AaAreA, AaLreA, AaLreB, or AaNsdD significantly decreased the fungal virulence. A comparative transcriptome analysis between the ∆AreA mutant and the wild-type strain revealed that the inactivation of AaAreA led to alterations in the expression of genes involved in a number of biological processes, including oxidoreductase activity, amino acid metabolism, and secondary metabolite biogenesis. Taken together, our findings revealed that GATA-coding genes play diverse roles in response to environmental stress and are important regulators involved in fungal development, conidiation, ROS detoxification, as well as pathogenesis. This study, for the first time, systemically underlines the critical role of GATA transcription factors in response to environmental stress and virulence in A. alternata. |
| Databáze: | OpenAIRE |
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