Date fruits inhibit hepatocyte apoptosis and modulate the expression of hepatocyte growth factor, cytochrome P450 2E1 and heme oxygenase-1 in carbon tetrachloride-induced liver fibrosis
| Autor: | Nouf M. Al-Rasheed, Hala A. Attia, Nawal M. Al-Rasheed, Raeesa A. Mohamad, Maha A. Al-Amin, Musaed Al Fayez |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Liver Cirrhosis
Male Physiology Liver fibrosis Immunoblotting CCL4 Caspase 3 Apoptosis Pharmacology Biology Immunoenzyme Techniques 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) medicine Animals Rats Wistar Carbon Tetrachloride Carbon Tetrachloride Poisoning Hepatocyte Growth Factor Plant Extracts Phoeniceae Cytochrome P-450 CYP2E1 General Medicine CYP2E1 Rats Heme oxygenase chemistry Biochemistry Gene Expression Regulation 030220 oncology & carcinogenesis Fruit Carbon tetrachloride Hepatocytes 030211 gastroenterology & hepatology Hepatocyte growth factor Heme Oxygenase-1 medicine.drug |
| Zdroj: | Archives of physiology and biochemistry. 123(2) |
| ISSN: | 1744-4160 |
| Popis: | Context: Date fruits have protective effects against liver fibrosis; however their anti-apoptotic effects have not been investigated.Objective: To investigate the modulating effects of date fruits on pro- and anti-apoptotic markers, cytochrome P450 2E1 (CYP2E1) and hepatocyte growth factor (HGF) in liver fibrosis. Materials and methods: Liver fibrosis was induced by injection of carbon tetrachloride (CCl4) for eight weeks. Date flesh extract (DFE) and pits extract (DPE) were taken daily concomitant with CCl4. Hepatocyte apoptosis was determined by measuring the expression of Fas, caspase-3, Bax, Bcl2 and hemeoxygenase-1 (HO-1). Hepatic levels of HGF and CYP2E1 were determined.Results: Treatment with DFE and DPE significantly attenuated the elevated levels of Fas, caspase 3, Bax and CYP2E1 induced by CCl4. In addition, they alleviated the reduction in Bcl2, HGF and HO-1, the cytoprotective and anti-apoptotic factors in liver. Conclusions DFE and DPE treatment can ameliorate liver fibrosis by inhibi... |
| Databáze: | OpenAIRE |
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