High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli
| Autor: | Bernhard O. Palsson, Eunju Lee, Suhyung Cho, Sun Chang Kim, Donghui Choe, Byung-Kwan Cho |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene Editing Cell division 030106 microbiology Biomedical Engineering Wild type General Medicine Gene Expression Regulation Bacterial Biology Cell morphology Biochemistry Genetics and Molecular Biology (miscellaneous) Phenotype Cell biology 03 medical and health sciences 030104 developmental biology Transcription (biology) CRISPR-Associated Protein 9 Doxycycline Escherichia coli RNA Messenger Microorganisms Genetically-Modified Gene Intracellular Genome Bacterial Abnormal cell morphology |
| Zdroj: | ACS synthetic biology. 7(4) |
| ISSN: | 2161-5063 |
| Popis: | Along with functional advances in the use of CRISPR/Cas9 for genome editing, endonuclease-deficient Cas9 (dCas9) has provided a versatile molecular tool for exploring gene functions. In principle, differences in cell phenotypes that result from the RNA-guided modulation of transcription levels by dCas9 are critical for inferring with gene function; however, the effect of intracellular dCas9 expression on bacterial morphology has not been systematically elucidated. Here, we observed unexpected morphological changes in Escherichia coli mediated by dCas9, which were then characterized using RNA sequencing (RNA-Seq) and chromatin immunoprecipitation sequencing (ChIP-Seq). Growth rates were severely decreased, to approximately 50% of those of wild type cells, depending on the expression levels of dCas9. Cell shape was changed to abnormal filamentous morphology, indicating that dCas9 affects bacterial cell division. RNA-Seq revealed that 574 genes were differentially transcribed in the presence of high expression levels of dCas9. Genes associated with cell division were upregulated, which was consistent with the observed atypical morphologies. In contrast, 221 genes were downregulated, and these mostly encoded proteins located in the cell membrane. Further, ChIP-Seq results showed that dCas9 directly binds upstream of 37 genes without single-guide RNA, including fimA, which encodes bacterial fimbriae. These results support the fact that dCas9 has critical effects on cell division as well as inner and outer membrane structure. Thus, to precisely understand gene functions using dCas9-driven transcriptional modulation, the regulation of intracellular levels of dCas9 is pivotal to avoid unexpected morphological changes in E. coli. |
| Databáze: | OpenAIRE |
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