Rapid robust separation of hydroxyproline and proline
| Autor: | Gene R. Nathans, Dennis R. Gere |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Chromatography Resolution (mass spectrometry) Proline Biophysics Cell Biology Chloroformate Biochemistry High-performance liquid chromatography Hydrolysate Amino acid chemistry.chemical_compound Hydroxyproline chemistry Derivatization Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | Analytical biochemistry. 202(2) |
| ISSN: | 0003-2697 |
| Popis: | The presence of hydroxyproline, and the determination of the ratio of the secondary amino acids proline to hydroxyproline, in amino acid hydrolysates specifically identifies collagen and collagen peptides. o-Phthalaldehyde, and then 9-fluorenylmethyl chloroformate, were used to carry out sequential prederivatization of amino acid hydrolysates in an in-line high-performance liquid chromatography sample loop. After derivatization, reversed-phase high-performance liquid chromatography with a C-18 ODS Hypersil cartridge column was used to resolve the hydroxyproline and proline from all primary amino acids, with resolution and detection of hydroxyproline and proline within 2.0 and 2.8 min, respectively, at concentrations in the range of picomoles per microliter of derivatized amino acid. The assay has a turnaround time of 10.75 min. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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