Functional Conservation of a Developmental Switch in Mammals since the Jurassic Age
| Autor: | Jayati Mookerjee-Basu, Suraj Peri, Emmanuelle Nicolas, Xiang Hua, Dietmar J. Kappes, Philip Czyzewicz, Lu Ge, Dai Zhongping, Juan I FuxmanBass, Qin Li, Albertha J.M. Walhout |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
T-Lymphocytes Biology 010603 evolutionary biology 01 natural sciences Genome Enhanceosome Conserved sequence Mice 03 medical and health sciences Silencer Elements Transcriptional Genetics Animals Humans Amino Acid Sequence Molecular Biology Discoveries Conserved Sequence Ecology Evolution Behavior and Systematics 030304 developmental biology Synteny Marsupial 0303 health sciences Gene targeting Opossums Evolution of mammals Silencer biology.organism_classification Biological Evolution Evolutionary biology Transcription Factors |
| Zdroj: | Molecular Biology and Evolution. 36:39-53 |
| ISSN: | 1537-1719 0737-4038 |
| DOI: | 10.1093/molbev/msy191 |
| Popis: | ThPOK is a “master regulator” of T lymphocyte lineage choice, whose presence or absence is sufficient to dictate development to the CD4 or CD8 lineages, respectively. Induction of ThPOK is transcriptionally regulated, via a lineage-specific silencer element, Sil(ThPOK). Here, we take advantage of the available genome sequence data as well as site-specific gene targeting technology, to evaluate the functional conservation of ThPOK regulation across mammalian evolution, and assess the importance of motif grammar (order and orientation of TF binding sites) on Sil(ThPOK) function in vivo. We make three important points: First, the Sil(ThPOK) is present in marsupial and placental mammals, but is not found in available genome assemblies of nonmammalian vertebrates, indicating that it arose after divergence of mammals from other vertebrates. Secondly, by replacing the murine Sil(ThPOK) in situ with its marsupial equivalent using a knockin approach, we demonstrate that the marsupial Sil(ThPOK) supports correct CD4 T lymphocyte lineage-specification in mice. To our knowledge, this is the first in vivo demonstration of functional equivalency for a silencer element between marsupial and placental mammals using a definitive knockin approach. Finally, we show that alteration of the position/orientation of a highly conserved region within the murine Sil(ThPOK) is sufficient to destroy silencer activity in vivo, demonstrating that motif grammar of this “solid” synteny block is critical for silencer function. Dependence of Sil(ThPOK) function on motif grammar conserved since the mid-Jurassic age, 165 Ma, suggests that the Sil(ThPOK) operates as a silenceosome, by analogy with the previously proposed enhanceosome model. |
| Databáze: | OpenAIRE |
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