A role for the inositol kinase Ipk1 in ciliary beating and length maintenance
| Autor: | Bruce Appel, Susan R. Wente, Bhaskarjyoti Sarmah, Virginia P. Winfrey, Gary E. Olson |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Protozoan Proteins
Microtubules Gene Expression Regulation Enzymologic Cell Line Animals Genetically Modified chemistry.chemical_compound Microtubule Intraflagellar transport Animals Humans Basal body Inositol Cilia Zebrafish Body Patterning Centrosome Multidisciplinary biology Vesicle Cilium Gene Expression Regulation Developmental Biological Transport Biological Sciences Zebrafish Proteins biology.organism_classification Cell biology Phosphotransferases (Alcohol Group Acceptor) chemistry |
| Zdroj: | Proceedings of the National Academy of Sciences. 104:19843-19848 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0706934104 |
| Popis: | Cilia project from cells as membranous extensions, with microtubule structural cores assembling from basal bodies by intraflagellar transport (IFT). Here, we report a ciliary role for the inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1) that generates inositol hexakisphosphate. In zebrafish embryos, reducing Ipk1 levels inhibited ciliary beating in Kupffer's vesicle and decreased ciliary length in the spinal canal, pronephric ducts, and Kupffer's vesicle. Electron microscopy showed that ciliary axonemal structures were not grossly altered. However, coincident knockdown of Ipk1 and IFT88 or IFT57 had synergistic perturbations. With GFP-Ipk1 enriched in centrosomes and basal bodies, we propose that Ipk1 plays a previously uncharacterized role in ciliary function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|