Multicenter Clinical Validation of PITX2 Methylation as a Prostate Specific Antigen Recurrence Predictor in Patients With Post-Radical Prostatectomy Prostate Cancer
| Autor: | Amy L. Lark, Esmeralda Castanos-Velez, Gunter Weiss, Chris H. Bangma, Thomas M. Wheeler, Arndt Hartman, Lionel L. Bañez, Stephen J. Freedland, Michael Ittmann, Leon Sun, John F. Madden, Geert J.L.H. van Leenders |
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| Přispěvatelé: | Pathology, Urology, Pediatrics |
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Oncology Biochemical recurrence medicine.medical_specialty Pathology Urology medicine.medical_treatment Adenocarcinoma Polymerase Chain Reaction Prostate cancer SDG 3 - Good Health and Well-being Prostate Internal medicine Biomarkers Tumor medicine Humans Promoter Regions Genetic Aged Proportional Hazards Models Retrospective Studies Homeodomain Proteins Prostatectomy business.industry Prostatic Neoplasms Cancer Methylation DNA Methylation Middle Aged Prostate-Specific Antigen Prognosis medicine.disease Survival Rate Log-rank test Prostate-specific antigen medicine.anatomical_structure Neoplasm Recurrence Local business Transcription Factors |
| Zdroj: | Journal of Urology, 184(1), 149-156. Elsevier Inc. |
| ISSN: | 0022-5347 |
| Popis: | Purpose: Radical prostatectomy is potentially curative in patients with clinically localized prostate cancer. However, biochemical recurrence affects 15% to 30% of men who undergo radical prostatectomy. We previously reported the prognostic potential of PITX2 gene promoter methylation using conventional assays. In the current study we validated PITX2 methylation status as a biochemical recurrence predictor after radical prostatectomy using a novel microarray based platform in a multi-institutional setting. Materials and Methods: PITX2 methylation status was assessed in formalin fixed, paraffin embedded prostatectomy tumor tissue samples from 476 patients from a total of 4 institutions on customized EpiChip (TM) PITX2 microarrays. Associations between PITX2 methylation and biochemical recurrence were assessed using the log rank test and Cox regression controlling for prostate cancer features. Results: On multivariate analysis men with high methylation status were at significantly higher risk for biochemical recurrence than those with low methylation status (HR 3.0, 95% CI 2.0-4.5, p |
| Databáze: | OpenAIRE |
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